【摘要】 为探索抗氧化酶活性水平及变化与种茧耐贮性之间的关系，以7个二化性品种和1个一化性品种柞蚕种茧为试材，比较分析雌、雄蛹在低温贮藏不同时间后血淋巴总SOD、POD、CAT抗氧化酶活性变化情况。结果表明：低温贮藏期间，3种抗氧化酶活性变化趋势在化性、品种、性别间较为相似，但活性水平均存在显著性差异，一化性品种总SOD、POD活性显著高于二化性品种，CAT活性显著低于二化性品种，各二化性品种之间同样具有显著性差异；相较于春季出库期，二化一放出库期各二化性柞蚕品种种茧蛹体血淋巴3种抗氧化酶活性均发生显著下降，其中，大9雌蛹总SOD、POD、CAT活性下降幅度最小，且始终处于较高水平，凤久和辽蚕527的雄蛹3种抗氧化酶活性较高，说明大9的雌蛹耐贮性最强，凤久和辽蚕527的雄蛹耐贮性最强。研究结果可为柞蚕种茧耐低温贮藏研究与二化一放品种选育提供理论依据。更多还原

【Abstract】 In order to explore the relationship between the levels and changes of antioxidant enzymes activity and the storability of tussah seed cocoons, the tussah seed cocoons of seven bivoltine tussah varieties and one univoltine tussah variety were taken as test materials to compare and analyze the activity changes of total SOD, POD and CAT in hemolymph of male and female tussah pupae under different low temperature storage time. The results showed that during the low temperature storage, the change trends of the activity of the three antioxidant enzymes were similar in their voltinism, varieties and genders, but there were significant differences in their activity levels. The total SOD and POD activity of the univoltine tussah variety were always significantly higher than those of bivoltine tussah varieties, and the CAT activity was significantly lower than those of bivoltine tussah varieties. There were also significant differences between bivoltine tussah varieties. Compared with the spring delivery period, the activity of three kinds of antioxidant enzymes in the hemolymph of the cocoon pupae of each bivoltine tussah variety during the delivery period of the single rearing of bivoltine tussah have significantly decreased, among which the activity of total SOD, POD and CAT of Da 9 female pupae decreased the least and remained at a high level all the time, and the activity of three antioxidant enzymes of Fengjiu and Liaocan 527 male individuals were relatively higher. It shows that the storage ability of Da 9 female pupae was the strongest, while that of the Fengjiu and Liaoning 527 male pupae was the strongest. The research results can provide a theoretical basis for the study on low temperature storage of tussah seed cocoons and the breeding of single rearing of bivoltine tussah varieties.更多还原