【摘要】 桑叶农药残留已成为影响蚕作安全的重要因素，严重威胁着蚕业生产的可持续健康稳定发展。为了减少蚕农因家蚕农药中毒造成的经济损失，文中以苯甲酰脲类杀虫剂氟铃脲为研究对象，采用高效液相色谱(high performance liquid chromatography, HPLC)法测定了微酸性电解水对溶液体系中氟铃脲的降解效果，发现贮存10 h的微酸性电解水与100μg/mL氟铃脲溶液混合处理30 min,降解率最高，达到79.07%。采用Fenton比色法测定微酸性电解水在贮存过程中产生羟自由基的能力，发现贮存10 h时产生羟自由基能力最强。采用生物学试验鉴定微酸性电解水对桑叶氟铃脲农药残留的去除效果，采用将微酸性电解水与氟铃脲溶液混合处理后喷洒桑叶饲喂家蚕、将氟铃脲污染的桑叶用微酸性电解水进行喷洒处理后饲喂家蚕以及将氟铃脲污染的桑叶用微酸性电解水进行浸泡处理后饲喂家蚕3种方式，5龄盛食蚕体质量增长率、熟蚕体质量、结茧率、化蛹率、全茧量和茧层量均显著提高，死蚕率和死笼率降低，且随着氟铃脲农药残留浓度降低，浸泡处理时间延长，效果改善越好。微酸性电解水能够在一定程度上降解桑叶上氟铃脲残留，减少桑叶氟铃脲含量，减轻家蚕农药中毒造成的危害。研究结果将为微酸性电解水在防范家蚕农药中毒中的应用提供参考。更多还原

【Abstract】 Pesticide residues in mulberry leaves have become an important factor affecting the safety of sericulture, which seriously threaten the stable, healthy and sustainable development of sericulture. In order to reduce the economic loss caused by pesticide poisoning in silkworm rearing, benzoylurea insecticide hexaflumuron was used as the research object in this paper. Firstly, the degradation effect of slightly acidic electrolyzed water(SAEW) on hexaflumuron solution was determined by high performance liquid chromatography. The results showed that the degradation rate of hexaflumuron was the highest, reaching 79.07%, when SAEW stored for 10 h was mixed with 100 μg/mL hexaflumuron solution for 30 min. It was also found that SAEW stored for 10 h had the strongest ability to produce hydroxyl radical by Fenton reaction colorimetry. Biological experiments were used to further identify the removal effect of SAEW on the pesticide residues of hexaflumuron in mulberry leaves. The results showed that three kinds of treatment methods, including mulberry leaves sprayed with SAEW and hexaflumuron mixed solution, hexaflumuron residual mulberry leaves sprayed with SAEW, and hexaflumuron residual leaves soaked with SAEW, could significantly improve the growth rate of body weight of 5th instar silkworm, mature silkworm body weight, cocooning rate, pupation rate, total cocoon weight and cocoon shell weight, and reduce the dead silkworm rate, and reduce the rate of dead worm cocoons. And with the decrease of hexaflumuron concentration and the extension of soaking treatment time, the removal effect of pesticide residues in mulberry leaves was better. SAEW could degrade the residual hexaflumuron in mulberry leaves, reduce the content of hexaflumuron in mulberry leaves and alleviate the harm caused by pesticide poisoning of silkworm to a certain extent. The results will provide reference for the application of SAEW in the prevention of silkworm pesticide poisoning.更多还原