【摘要】 积雪草提取物通常被用作化妆品原料，有美白、抗氧化等功效，发挥此功效的主要物质有积雪草苷、羟基积雪草苷。采用高效液相色谱-蒸发光散射检测（HPLC-ELSD）技术，建立了测定积雪草中积雪草苷和羟基积雪草苷含量的方法。选用Unitary C₁₈色谱柱（4.6×250 mm,5μm），以乙腈-水为流动相，梯度洗脱，流速1 mL/min，柱温为30℃，采用Agilent 1260 InfinityⅡ蒸发光散射检测器（漂移管温度70℃，载气流速1.6 L/min）。研究表明，积雪草苷和羟基积雪草苷质量浓度分别在100～702 mg/L,105～738 mg/L范围内，峰面积与质量浓度线性关系良好，相关系数分别为0.999 6和0.999 7。积雪草苷和羟基积雪草苷平均加样回收率分别为101.20%,98.30%。相对标准偏差（RSD）均在3%以内。方法简单，准确度高，分离效果好，干扰少，可用于积雪草中积雪草苷和羟基积雪草苷的含量测定。更多还原

【Abstract】 Centella asiatica(L.) Urban extract is usually used as a cosmetic raw material, which has whitening, antioxidant and other effects. The main substances to play this effect are asiaticoside and madecassoside. In this paper, a high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD) technique was used to determine the content of asiaticoside and madecassoside in Centella asiatica(L.) Urban. Unitary C18 column(4.6×250 mm, 5 μm) with acetonitrile-water as the mobile phase was used for gradient elution at a flow rate of 1 mL/min. Column temperature was 30 ℃. Agilent 1260 Infinity Ⅱ detector(drift tube temperature was 70 ℃) was used for column elution with the carrier gas flow rate at 1.6 L/min. The results show that the concentrations of asiaticoside and madecassoside are 100-702 mg/L and 105-738 mg/L, respectively. The linear relationship between peak area and concentration is good, and the correlation coefficients are 0.999 6 and 0.999 7, respectively. The average recoveries of asiaticoside and madecassoside are 101.20% and 98.30%, respectively. The relative standard deviations(RSD) are all within 3%. The method is simple, accurate, effective and less interference, and can be used for the determination of asiaticoside and madecassoside in Centella asiatica(L.) Urban.更多还原