【摘要】 使用液晶乳化剂与传统油包水乳化剂通过两步法制备了一种多重乳状液，显微镜观察结果显示，油水界面存在层状液晶，即该多重乳为水包液晶包油包多元醇的结构（P/O/LC/W）。系统考察了液晶乳化剂种类以及乳化温度、均质速率对多重乳微观结构的影响。实验结果表明，当液晶乳化剂使用IST-LC001，制备工艺控制乳化温度60～75℃，油包醇乳液制备阶段均质速率7 000～8 400 r/min，水包液晶包油包醇乳液制备阶段均质速率4 400～6 000 r/min时，可以获得理想的多重乳。使用该多重乳分别包覆活性物质白藜芦醇与紫草素，实验结果显示，该乳液不仅可以解决难溶于水、油的功效成分在配方中的运用，有效包覆、缓释活性物质，还能够掩盖包裹成分的颜色、改善产品的肤感。乳液稳定性良好，在医药、食品、化妆品等领域都有广阔的应用价值。更多还原

【Abstract】 A type of polyol-in-oil-in-liquid crystal-in-water(P/O/LC/W) multiple emulsion was prepared by two-step method with liquid crystal emulsifier and traditional emulsifier. The samples were characterized by microscopy. The effects of different liquid crystal emulsifiers, emulsification temperature and homogenization rate on the microstructure of P/O/LC/W multiple emulsion were studied. The steps of preparation process were as follows: The emulsification temperature was 60-75 ℃; the homogenization rate of polyol in oil was 7 000-8 400 r/min and homogenization time was ～6 min; the homogenization rate of polyol in oil in liquid crystal in water was 4 400-6 000 r/min and homogenization time was ～7 min. The emulsion was put at room temperature for 28 days, and multiple structures and liquid crystal structures could still be observed, and the liquid crystal became brighter. The components of the formula were glycerol(polyol phase), squalane(oil phase), P135(hydrophobic emulsifier), and IST-LC001(liquid crystal emulsifier). The ideal emulsion structure could thus be obtained. The formula was used to encapsulate active substances. Resveratrol was used as the target molecule for sustained-release experiment. The results of dialysis experiments in vitro showed that the P/O/LC/W multiple emulsion had a remarkable slow-release performance. The release ratio of target molecule was up to 83% after 10 h. When shikonin was used as the target molecule, it was proved that the P/O/LC/W multiple emulsion could mask the color of the target molecule. Compared with traditional emulsion, the color of P/O/LC/W multiple emulsion was lighter. Rheological measurement showed that the viscosity of the emulsion decreased with the increase of shear stress, indicative of positive thixotropy. Within the investigated range, the storage modulus of the emulsion was always higher than that of loss modulus, exhibiting elastic properties. The internal structure of the emulsion was stable and the emulsion could bring good skin feel to skin care products. The experimental results showed that, the P/O/LC/W multiple emulsion could not only solve the problems in application of insoluble functional ingredients in formulations, but also effectively deliver these active substances and achieve slow-release. The emulsion could also mask the color of introduced ingredients and improve the skin feel of products. The P/O/LC/W multiple emulsion has wide application value in the fields of medicine, food, cosmetics, etc.更多还原