【摘要】 目的 探讨丝胶对链脲佐菌素（STZ）致损伤大鼠胰岛素瘤细胞（INS-1细胞）的保护作用。方法 实验以体外培养的INS-1细胞为研究对象，随机分为5组：正常对照组（常规条件培养细胞）、模型组（培养基中含10mmol/L的STZ）、丝胶低、中、高浓度组（培养基中分别含10mmol/L的STZ，及150μg/mL、300μg/mL、600μg/mL的丝胶），每组细胞药物作用时间均为24h。蛋白印迹和实时荧光定量PCR法检测各组细胞BCL-2与P53蛋白及mRNA的表达情况。流式细胞术检测各组细胞的凋亡情况。结果 与正常对照组相比，在模型组中，INS-1细胞中BCL-2蛋白和mRNA的表达显著减少，P53蛋白和mRNA的表达显著增加，早期凋亡率显著升高（P<0.05）。与模型组相比，丝胶各组INS-1细胞BCL-2蛋白与mRNA的表达均显著增多，P53的蛋白与mRNA的表达均显著下降，早期凋亡率显著下降（P<0.05）;3个丝胶组两两比较差异均有统计学意义（P<0.05）。结论 丝胶对STZ致损伤INS-1细胞发挥保护作用的机制与调控凋亡蛋白，抑制INS-1细胞凋亡有关。更多还原

【Abstract】 Objective To explore the protective effects of sericin on streptozotocin(STZ)-induced injury rats’ INS-1 cells.Methods INS-1 cells were cultured in vitro and randomly divided into five groups. Normal control group(culture under conventional conditions), model group(10mmol/L STZ), low sericin group(STZ+150μg/mL sericin), medium sericin group(STZ+300μg/m L sericin), high sericin group(STZ+600μg/mL sericin). The cells in five groups were cultured respectively with corresponding drugs for 24h. The protein and mRNA expressions of BCL-2 and P53 were detected by Western blotting and real-time PCR. Cell apoptosis was detected by flow cytometry. Results Compared with the normal control group, the expression of BCL-2 protein and mRNA of INS-1 cells in the model group were remarkably decreased, while the expression of P53 protein and mRNA were remarkably increased(P<0.05), as well as the early apoptosis rate was remarkably increased(P<0.05). Compared with the model group, the expression of BCL-2 protein and mRNA in 3 sericin groups were remarkably increased, while the expression of P53 protein and mRNA were remarkably decreased(P<0.05), and the early apoptosis rate was remarkably decreased(P<0.05). Moreover, there were statistically remarkable differences among the 3 sericin groups(P<0.05). Conclusion The protective effects of sericin on STZ-induced injury INS-1 cells is related to the regulation of apoptotic proteins and inhibition of INS-1 cell apoptosis.更多还原