【摘要】 以组织型纤溶酶原激活剂-抑制剂复合物（t-PAIC）为免疫原制备和筛选了一对特异性单克隆抗体，并建立了一种检测人体血浆t-PAIC的磁微粒化学发光免疫检测方法。通过与参比方法比对，选定了反应速度更快的一步法作为反应模式，然后对t-PAIC检测方法的检测性能进行了评估。结果显示：该方法的空白限为0.43 ng/mL，检出限为0.91 ng/mL；线性范围为0.91～100ng/mL；重复性CV小于4%，精密度CV小于3%；样本回收率在100%～110%之间；常见干扰物对检测结果无明显影响。构建了t-PAIC检测方法的参考区间，健康成年男性参考范围为1.85～15.91 ng/mL，健康成年女性参考范围为1.90～9.43 ng/mL。t-PAIC检测方法反应快速，在血栓疾病诊断中有重要的应用价值。更多还原

【Abstract】 A pair of specific monoclonal antibodies were prepared and screened by using tissue type plasminogen activator inhibitor complex(t-PAIC) as immunogen, and then a magnetic particle chemiluminescence immunoassay method was established for the detection of human plasma t-PAIC. In comparison with the reference method, the one-step method with a faster reaction speed was finally selected as the reaction mode. Then the performance of the method was estimated, the results showed that the limit of blank(LOB) was 0.43 ng/mL, the limit of detection(LOD) was 0.91 ng/mL, and the linear range was 0.91～100 ng/mL. The coefficient of variance(CV) of repeatability was less than 4%, and the CV of precision was less than 3%. The sample recovery rate was between 100%～110%, and the common interfering substances had no obvious influence on the detection results. Finally, the reference interval of the method was constructed. The reference range for healthy adult males was 1.85～15.91 ng/mL, and that of 1.90～9.43 ng/mL for healthy adult women. The method has the advantage of rapid reaction, which is of important application value in the diagnosis of thrombotic diseases.更多还原