【摘要】 目的:研究骨碎补总黄酮对亚硝酸钠模型小鼠学习记忆障碍的改善作用。方法:昆明雄性小鼠随机分为空白组、亚硝酸钠模型组100 mg/（kg·d）、抗脑衰胶囊组585 mg/（kg·d）、骨碎补总黄酮97.5 mg/（kg·d）、骨碎补总黄酮组+ER阻断剂组97.5 mg/（kg·d）+0.072 mg/（kg·d）。观察小鼠的学习记忆能力，HE染色观察海马病理形态；Western blot法观察海马区淀粉样蛋白前体蛋白（amyloid precursor protein, APP）、淀粉样前体蛋白β-分泌酶（amyloid precursor protein β-secretory enzyme, BACE1）、过度磷酸化tau蛋白(over-phosphorylated tau protein, P-Tau³⁹⁶)和细胞周期素依赖蛋白激酶5（cyclin-dependent protein kinase 5,CDK5）蛋白含量。结果:模型组学习行为能力降低，海马区颗粒细胞明显坏死，海马区APP、BACE1、P-Tau³⁹⁶和CDK5表达水平升高（P<0.01）。骨碎补总黄酮能够改善模型小鼠的行为学表现，降低APP、BACE1、P-Tau³⁹⁶和CDK5的表达（P<0.01）。ER阻断剂能够反转骨碎补总黄酮在以上指标蛋白中的作用。结论:骨碎补总黄酮对亚硝酸钠模型小鼠学习记忆能力具有改善作用，此效应与调节APP代谢途径相关蛋白的影响相关。更多还原

【Abstract】 Objective:To study the effect of total flavonoids of Rhizoma Drynariae（TFRD）on learning and memory impairment of sodium nitrite model mice. Methods:KM mice were randomly divided into the blank group, sodium nitrite model group 100 mg/（kg·d）, Kangnaoshuai capsule group 585 mg/（kg·d）, TFRD group 97.5 mg/（kg·d）, and the TFRD+ER blocker group 97.5 mg/（kg·d）+0.072 mg/（kg·d）. The learning and memory ability of mice were observed. Morphology of hippocampus was detected with HE staining. The contents of amyloid precursor protein（APP）, amyloid precursor protein β-secretase（BACE1）, over-phosphorylated tau protein(P-Tau³⁹⁶), cyclin-dependent protein kinase 5（CDK5）, estrogen receptor β（ERβ） and phosphorylated p38（p-p38） in hippocampus were tested with Western blot. Results:The learning ability of mice in the model group decreased significantly. Granular cells in hippocampus were obviously necrotic. The expression levels of APP, BACE1, P-Tau³⁹⁶, CDK5 and P-P38/P38 in hippocampus increased significantly（P<0.01）, while the expression level of ERβ decreased significantly（P<0.01）. TFRD could improve the behavioral performance of mice in different models, decrease the expression levels of APP、BACE1、P-Tau³⁹⁶、CDK5 and P-P38/P38（P<0.01）, and increase the expression levels of ERβ. ER blocker could reverse the effect of TRFD on the above proteins. Conclusion:TFRD could improve learning and memory ability of mice in sodium nitrite model, which may due to the effect of TFRD on regulating proteins related to APP metabolic pathway.更多还原