【摘要】 目的:探讨淫羊藿苷(icariin, ICA)对成牙骨质细胞(OCCM-30)体外增殖及成骨分化能力的影响。方法:通过体外培养OCCM-30细胞，加入不同浓度(10、20、40μmol/L) ICA,倒置显微镜下观察细胞形态。设置10μmol/L ICA实验组与空白对照组处理，MTT法连续7 d检测OCCM-30细胞增殖率。通过Real-time PCR研究10μmol/L ICA作用于OCCM-30细胞后(1、4、7 d)成骨相关因子OCN和Runx-2的表达。结果:与对照组相比，加入ICA后OCCM-30细胞形态无显著变化，MTT结果显示10μmol/L ICA明显促进OCCM-30细胞增殖(P<0.05)。Real-time PCR结果显示实验组中成骨相关因子OCN和Runx-2的表达显著增高，与对照组相比较差异具有统计学意义(P<0.05)。结论:适宜浓度的ICA可有效促进OCCM-30的体外增殖及向成骨分化。更多还原

【Abstract】 Objective:To investigate the effects of Icariin(ICA) on the proliferation and osteogenic differentiation of cementoblasts(OCCM-30) in vitro. Methods:OCCM-30 cells were cultured in vitro and added with different concentrations(10, 20, 40 μmol/L) of ICA and observed the cell morphology under inverted microscope. OCCM-30 cells was cultured in vitro and treated with blank control group and 10 μmol/L ICA group. The proliferation rate of OCCM-30 cells was detected by MTT method for 7 days. Real-time PCR was used to study the expression of osteogenic-related factors OCN and Runx-2 after 10 μmol/LICA was applied to OCCM-30 cells(1 d, 4 d, 7 d). Result:The results showed that there was no significant change in the morphology of OCCM-30 cells after adding ICA. MTT results showed that 10 μmol/L ICA significantly promoted the proliferation of OCCM-30 cells(P<0.05). Real-time PCR results showed that the expression of osteogenic related factors OCN and Runx-2 in the experimental group was significantly higher than that in the control group(P<0.05). Conclusion:Appropriate concentration of ICA can effectively promote the proliferation and osteogenic differentiation of OCCM-30 cells in vitro.更多还原