【摘要】 副干酪乳酸杆菌ET-22是一种具有缓解炎症、调节免疫功能的益生菌，为探究其对口腔疾病的改善作用及机制，采用甲基紫精颊膜注射法建立金黄地鼠口腔溃疡模型，在造模前，分别以灌胃的方式连续给予金黄地鼠剂量为1×10⁹ CFU·mL^-1·d^-1的ET-22活菌以及对应的ET-22灭活菌和ET-22发酵液，研究ET-22对地鼠口腔黏膜结构形态和炎性因子表达水平影响。口腔黏膜的组织病理切片观察和评分结果表明，ET-22活菌、灭活菌及其发酵液均能改善地鼠口腔黏膜的上皮完整性和炎性细胞浸润，ET-22活菌预防口腔溃疡的效果最佳，ET-22灭活菌及其发酵液的预防效果次之，但它们的预防效果均优于维生素C。机制研究发现：ET-22可通过下调NF-κB的表达抑制促炎细胞因子IL-6和IL-1β的分泌，降低基质金属蛋白酶MMP-9的表达，减轻口腔黏膜的炎症浸润与转移；ET-22可以调节口腔的菌群组成，抑制口腔疾病相关的有害菌。研究表明，ET-22可降低口腔溃疡的发病风险，对预防口腔溃疡具有积极作用。更多还原

【Abstract】 Lactobacillus paracasei ET-22 is a kind of probiotic that can relieve intestinal inflammation and regulate immune functions, but the effects on oral diseases and its mechanism need to be further studied. In this study, an oral ulcer model based on golden hamster was established by buccal injection of methyl viologen, and the effects of ET-22 on the phenotype of oral ulcer and the inflammatory factors expression level were studied through previously treating hamsters with 1×10~9 CFU/mL/day of live ET-22, inactivated ET-22 and fermented broth of ET-22. The results based on histopathological section observation and score of oral mucosa showed that the intervention of live ET-22, inactivated ET-22 and its fermented broth could improve the epithelial integrity and inflammatory cell infiltration of oral mucosa in oral ulcer model hamsters. And the live ET-22 had the best performance on prevention of oral ulcer, followed by inactivated ET-22 and fermented broth of ET-22, but their effects were all better than the effect of vitamin C intervention. On the one hand, ET-22 could inhibit the secretion of pro-inflammatory cytokines IL-6 and IL-1β by down-regulating the expression of NF-κB, thereby reduced the expression of matrix metalloproteinase MMP-9 and alleviated the inflammatory infiltration and metastasis of oral mucosa. On the other hand, ET-22 could regulate oral microbiota composition and inhibit harmful bacteria related to oral diseases. The study indicated that ET-22 could decrease the risk of oral ulcer and play a positive role in the prevention of oral ulcer.更多还原