【摘要】 目的：探讨人参皂苷Rh2对糖尿病肾病变（DKL）大鼠肾脏损伤的保护作用，并阐明其作用机制。方法：通过高脂高能量饲料配以链脲佐菌素（STZ）腹腔注射方法制备DKL大鼠模型。将30只SD成模大鼠随机分为模型组、低剂量(10 mg·kg^-1) Rh2组和高剂量(20 mg·kg^-1) Rh2组，每组10只，另选取10只SD大鼠作为正常对照组。检测各组大鼠体质量和肾脏质量，并计算肾指数，干预8周后全自动分析仪检测各组大鼠血清肌酐（Scr）、尿素氮（BUN）和24 h尿蛋白（UP）水平，酶联免疫吸附测定（ELISA）法检测各组大鼠肾组织中Ⅳ型胶原（ColⅣ）水平，Western blotting法检测各组大鼠肾组织中转化生长因子β1 （TGF-β1）、Smad3和Smad7蛋白表达水平。结果：与正常对照组比较，模型组大鼠体质量降低（P<0.01），肾脏质量和肾指数明显升高（P<0.01），血清中Scr、BUN和24 h UP水平均升高（P<0.01），肾组织中ColⅣ水平升高（P<0.01），肾组织中TGF-β1和Smad3蛋白表达水平升高（P<0.01），而肾组织中Smad7蛋白表达水平降低（P<0.01）。与模型组比较，低和高剂量Rh2组大鼠体质量升高（P<0.05或P<0.01），肾脏质量和肾指数均降低（P<0.05或P<0.01），血清中Scr、BUN和24 h UP水平均明显降低（P<0.05或P<0.01），肾组织中ColⅣ水平降低（P<0.01），肾组织中TGF-β1和Smad3蛋白表达水平降低（P<0.01），肾组织中Smad7蛋白表达水平升高（P<0.01）。结论：人参皂苷Rh2可抑制DKL大鼠肾脏纤维化，改善肾功能，对DKL大鼠肾脏有保护作用，其机制可能与Rh2调节TGF-β1/Smads信号通路表达有关。更多还原

【Abstract】 Objective:To discuss the protective effect of ginsenoside Rh2 on kidney injury in the rats with diabetic kidney lesions（DKL）,and to clarify its mechanism. Methods:The DKL rat model was established by using the high-fat and high-energy diet combined with intraperitoneal injection of streptozotocin（STZ）. Thirty SD model rats were randomly divided into model group, low dose(10 mg·kg^-1) of Rh2 group and high dose(20 mg·kg^-1) of Rh2 group, and there were 10 rats in each group;other ten SD rats were regared as normal control group. The body weights and kidney weights of the rats in various groups were detected,and the kidney index was caculated;after intervented for 8 weeks, automatic analyzer was used to detect the levels of serum creatinine（Scr）, urea nitrogen（BUN） and 24 h urinary protein（UP）in serum of the rats in various groups;enzyme linked immunosorbent assay（ELISA） method was used to detect the levels of collagen type Ⅳ（Col Ⅳ） in kidney tissue of the rats in various groups; Western blotting method was used to detect the expression levels of transforming growth factor-β1（TGF-β1）,Smad3 and Smad7 proteins in kidney tissue of the rats in various groups.Results:Compared with normal control group, the body weight of the rats in model group was decreased（P<0. 01）,the kidney weight and kidney index were significantly increased（P<0. 01）, the levels of Scr, BUN and 24 h UP in serum were increased（P<0. 01）, the level of Col Ⅳ in kidney tissue was increased（P<0. 01）, the expression levels of TGF-β1 and Smad3 proteins in kidney tissue were increased（P<0. 01）, and the expression level of Smad7 protein in renal tissue was decreased（P<0. 01）. Compared with model group,the body weight of the rats in low and high doses of Rh2 groups were inecreased（P<0. 05 or P<0. 01）, the kidney weight and kidney index were decreased（P<0. 05 or P<0. 01）,the levels of Scr, BUN and 24 h UP in serum were significantly decreased（P<0. 05 or P<0. 01）, the level of Col Ⅳ in kidney tissue was decreased（P<0. 01）, the expression levels of TGF-β1 and Smad3 proteins in kidney tissue were decreased（P<0. 01）, and the expression level of Smad7 protein in kidney tissue was increased（P<0. 01）. Conclusion:Ginsenoside Rh2 can inhibit the kidney fibrosis and improves the kidney function in the DKL rats, and has a protective effect on the kidney of DKL rats; its mechanism may be related to regulating the expression of TGF-β1/Smads signaling pathway.更多还原