【摘要】 目的：探讨微小RNA-491-5p (miR-491-5p)过表达对人鼻咽癌HONE-1细胞增殖和迁移的影响，为研究鼻咽癌的发病机制和靶向治疗提供依据。方法：将人鼻咽癌HONE-1细胞分为对照组（转染对照质粒）和miR-491-5p组（转染miR-491-5p质粒）。采用荧光显微镜观察2组鼻咽癌HONE-1细胞转染效率，CCK-8法检测2组鼻咽癌HONE-1细胞增殖活性，Transwell小室实验检测2组鼻咽癌HONE-1细胞的迁移细胞数，实时荧光定量PCR (RT-qPCR)法检测2组鼻咽癌HONE-1细胞中波形蛋白和上皮钙黏素mRNA表达水平，Western blotting法检测2组鼻咽癌HONE-1细胞中波形蛋白和上皮钙黏素蛋白表达水平。结果：与对照组比较，作用24、48和72 h时miR-491-5p组鼻咽癌HONE-1细胞增殖活性（t=2.832,P=0.047 3;t=4.522,P=0.001 4;t=9.308,P<0.01）和迁移细胞数（t=9.639, P<0.01）明显降低；与对照组比较，miR-491-5p组鼻咽癌HONE-1细胞中波形蛋白mRNA (t=7.535,P=0.001 7)和蛋白（t=7.219,P=0.018 7）表达水平明显降低，上皮钙黏素mRNA (t=4.88, P=0.0395)和蛋白（t=5.754, P=0.0289）表达水平明显升高。结论：MiR-491-5p过表达可以抑制人鼻咽癌细胞的增殖、迁移和上皮-间质转化（EMT）。更多还原

【Abstract】 Objective:To discuss the effect of microRNA-491-5p(miR-491-5p) over-expression on the proliferation and migration of the human nasopharyngeal carcinoma HONE-1 cells, and to provide the evidence for the study of the pathogenesis and targeted therapy of nasopharyngeal carcinoma. Methods:The human nasopharyngeal carcinoma HONE-1 cells were divided into control group(transfected with control plasmid) and miR-491-5p group(transfected with miR-491-5p plasmid). The transfection efficiencies of the HONE-1 cells in two groups were observed under fluorescence microscope; the proliferation activities of the HONE-1 cells in two groups were detected by CCK-8 assay; the number of migration HONE-1 cells in two groups were detected by Transwell chamber assay;the expression levels of Vimentin and E-cadherin mRNA in the HONE-1 cells in two groups were detected by real-time fluorescence quantitative PCR(RTqPCR)method; the expression levels of Vimentin and E-cadherin proteins in the HONE-1 cells in two groups were detected by Western blotting method. Results:Compared with control group, the proliferation activities(t=2. 832, P=0. 0473; t=4. 522, P=0. 0014; t=9. 308, P<0. 01) and number of migration HONE-1 cells(t=9. 639, P<0. 01) in miR-491-5p group at 24,48,and 72 h after treatment were significantly decreased; compared with control group, the expression levels of Vimentin mRNA(t=7. 535, P=0. 0017) and protein(t=7. 219, P=0. 0187) in the HONE-1 cells in miR-491-5p group were significantly decreased, and the expression levels of E-cadherin mRNA(t=4. 88, P=0. 0395) and protein(t=5. 754, P=0. 0289) were significantly increased. Conclusion:Over-expression of miR-491-5p can inhibit the proliferation, migration, and epithelial-mesenchymal transition(EMT) of the human nasopharyngeal carcinoma cells.更多还原