【摘要】 【目的】通过分析木屑与5种不同农业秸秆的营养成分及其对大球盖菇菌丝生长的影响，为木屑秸秆在大球盖菇上的应用提供依据。【方法】对以木屑和5种秸秆的单一培养基以及复合培养基培养的大球盖菇菌丝的生长情况以及酶活进行了观察记录与测定。【结果】木屑、稻壳、稻草、玉米芯、玉米秸秆、棉籽壳的含氮量范围在0.48%～1.21%;含碳量范围在47.22%～54.35%,玉米芯碳氮比最大，木屑的碳氮比最小；玉米秸秆的纤维素含量最高，玉米芯半纤维素含量最高，木屑木质素含量最高。在木屑与5种农业秸秆单一基质培养基的基础上添加氮源和微量元素可以显著增加大球盖菇菌丝干质量及菌丝密度。木屑、玉米芯、稻草为底物时大球盖菇具有较高的木质纤维素酶活性。在木屑与5种农业秸秆配比的复合培养基上，木屑比例越高，大球盖菇菌丝干质量越大，复合培养基添加氮源和微量元素后则提高菌丝干质量3倍以上。【结论】大球盖菇在复合基质培养基中比在单一基质培养基中生长更好，在氮源和微量元素满足时菌丝生长更好。更多还原

【Abstract】 [Objective]By analyzing the nutritional components of sawdust and five different agricultural straws and their effects on the mycelial growth of Stropharia rugosoannulata, the basis for the application of sawdust straw in Stropharia rugosoannulata was provided. [Methods]The mycelium growth and enzyme activity of Stropharia rugosoannulata cultured in single medium with sawdust and five kinds of straw and compound medium were observed, recorded and determined.[Results]The nitrogen content of sawdust, rice husk, straw, corn cob, corn straw and cottonseed hull ranged from 0.48% to 1.21%. The carbon content ranged from 47.22% to 54.35%, the carbon-nitrogen ratio of corncob was the largest, and the carbon-nitrogen ratio of sawdust was the smallest. Corn straw had the highest cellulose, corncob had the highest hemicellulose, and sawdust had the highest lignin content. Adding nitrogen source and trace elements on the basis of sawdust and five kinds of agricultural straw single matrix medium can significantly increase the mycelial dry weight and mycelial density of Stropharia rugosoannulata. Stropharia rugosoannulata had higher lignocellulase activity when sawdust, corncob and straw were used as substrates. On the composite medium of sawdust and five kinds of agricultural straw, the higher the proportion of sawdust, the greater the dry weight of Stropharia rugosoannulata mycelium. After adding nitrogen source and trace elements to the composite medium, the dry weight of mycelium was increased by more than three times. [Conclusion]The growth of Stropharia rugosoannulata in compound matrix medium was better than that in single matrix medium. The mycelial growth was better when the nitrogen source and trace elements were satisfied.更多还原