【摘要】 目的 探讨无齿E3泛素蛋白连接酶同源物（DTL）在食管鳞状细胞癌（ESCC）中的表达及与预后的关系。方法 收集TIMER2数据库资料，分析DTL在食管癌组织中的mRNA表达水平。采用免疫组化染色法检测95例ESCC组织和癌旁正常组织中DTL的蛋白表达情况，分析DTL表达情况与ESCC患者临床特征的关系，并分析ESCC患者预后的影响因素。结果 TIMER2数据库分析结果显示，食管癌组织中DTL mRNA表达水平高于正常食管组织，差异有统计学意义（P﹤0.05）。ESCC组织中DTL阳性表达率为56.8%(54/95)，明显高于癌旁正常组织的35.8%(34/95)，差异有统计学意义（P﹤0.01）。不同病灶数目及组织学分级ESCC患者的ESCC组织中DTL表达情况比较，差异均有统计学意义（P﹤0.05）。DTL阳性表达的ESCC患者总生存期明显短于DTL阴性表达患者，差异有统计学意义（P﹤0.01）。多因素分析结果显示，DTL阳性表达是ESCC患者预后的独立危险因素（P﹤0.01）。结论 DTL在ESCC中高表达，且与ESCC的发生发展密切相关，可能是ESCC潜在的预后分子标志物。更多还原

【Abstract】 Objective To investigate the expression of denticleless E3 ubiquitin protein ligase homolog(DTL) in esophageal squamous cell carcinoma(ESCC) and its relationship with prognosis. Method TIMER2 database was used to analyze the mRNA expression of DTL in esophageal cancer tissues. Immunohistochemistry(IHC) staining was used to detect the protein expression of DTL in 95 cases of ESCC tissues and adjacent normal tissues, and to analyze the relationship of DTL expression with clinical features of ESCC patients and influencing factors for prognosis of ESCC patients.Result The analysis results of TIMER2 database showed that the expression of DTL mRNA was up-regulated in esophageal cancer tissues than that in normal esophageal tissues(P<0.05); the positive expression rate of DTL in ESCC tissues was higher than that in adjacent normal tissues [56.8%(54/95) vs 35.8%(34/95), P<0.01]; there were significant differences for DTL expression in ESCC tissues in patients with different number of lesions and different histological grades(P<0.05); the overall survival(OS) of ESCC patients with DTL positive expression was shorter than that of patients with DTL negative expression(P<0.01); multivariate analysis showed that DTL positive expression was independent risk factor for prognosis of ESCC patients(P<0.01). Conclusion DTL is highly expressed in ESCC and closely related to the occurrence and development of ESCC, which may be a potential prognostic molecular marker of ESCC.更多还原