【摘要】 本研究以济薯25为材料，克隆得到IbbZIP22基因。该基因编码区全长1 368 bp,编码455个氨基酸。序列和结构分析发现该基因编码的蛋白上有bZIP＿plant＿RF2保守结构域，推测其属于RF2类bZIP转录因子；对IbbZIP22蛋白进行亚细胞定位预测，推测该蛋白位于细胞核中；系统发育分析表明，IbbZIP22蛋白与三裂叶薯中的ItRF2a-like蛋白亲缘关系最近。采用实时定量PCR技术对济薯25和徐紫薯8号不同部位的IbbZIP22基因表达模式进行分析，发现IbbZIP22基因均在两个品种块根中表达量最高，且济薯25块根中的表达量显著高于徐紫薯8号，推测该基因参与淀粉调控。利用同源克隆技术，从济薯25基因组DNA中克隆IbbZIP22基因的启动子序列，该序列中除包含CAAT-box、TATA-box等核心启动子元件外，还存在多个参与光响应、抗逆和激素应答等元件。本研究结果为探究甘薯抗逆和淀粉调控机制提供了理论依据。更多还原

【Abstract】 In this study, the IbbZIP22 gene was cloned from Jishu 25. The IbbZIP22 gene coding region was 1 368 bp in full length and encoded 455 amino acids. Sequence and structural analysis showed that the bZIP＿plant＿RF2 conserved domain was found in the protein encoded by this gene, which was speculated to be a bZIP transcription factor of RF2 class. The IbbZIP22 protein was predicted to be located in the nucleus by subcellular localization. Phylogenetic analysis showed that IbbZIP22 protein was most closely related to ItRF2a-like protein in Ipomoea triloba. The expression pattern of IbbZIP22 gene in different parts of Jishu 25 and Xuzishu 8 was analyzed by real-time PCR. It was found that the expression level of IbbZIP22 gene in tuber root was the highest in both varieties, and the expression level in tuber root of Jishu 25 was significantly higher than that of Xuzishu 8, speculating that this gene was involved in starch regulation. The promoter sequence of IbbZIP22 gene was cloned from Jishu 25 genomic DNA by homologous cloning technique. In addition to the core promoter elements such as CAAT-box and TATA-box, the sequence also contained several elements involved in light response, stress resistance and hormone response. These results could provide a theoretical basis for exploring the mechanisms of resistance to stress and starch regulation in sweet potato.更多还原