【摘要】 目的：探讨非小细胞肺癌(NSCLC)患者血清miR-146a与肿瘤坏死因子α(TNF-α)、T调节细胞(Treg)的相关性及miR-146a对患者预后的预测价值。方法：选择2020年6月—2021年11月在沧州市中心医院治疗的中晚期NSCLC患者162例。采用荧光定量PCR法检测患者血清中miR-146a的相对表达水平，采用酶联免疫法检测患者血清中TNF-α浓度，采用流式细胞术检测患者静脉血中的Treg细胞比例。采用Pearson相关分析miR-146a相对表达水平与TNF-α浓度、Treg细胞比例的相关性，采用多因素logistic回归分析miR-146a相对表达水平是否为NSCLC患者的预后危险因素，采用ROC曲线检测miR-146a对患者预后的预测效能。结果：NSCLC患者血清中miR-146a的相对表达水平为1.48±0.18,TNF-α的浓度为(29.46±4.19)μg/L，静脉血中Treg细胞比例为(4.03±0.85)%,且miR-146a相对表达水平与TNF-α浓度呈正相关(r=0.651,P<0.01)，与Treg比例呈负相关(r=-0.676,P<0.01)。miR-146a相对表达水平和TNF-α浓度为预后危险因素(OR分别为1.95和1.39，均为P<0.01),Treg细胞比例为NSCLC患者死亡的保护因素(OR=0.53;P<0.01)。miR-146a相对表达水平的AUC为0.959,95%CI(0.934,0.985),P<0.01。结论：NSCLC患者的血清miR-146a相对表达水平与TNF-α浓度呈正相关，与Treg细胞比例呈负相关，是NSCLC患者死亡的危险因素，对NSCLC预后有较高的预测价值。更多还原

【Abstract】 OBJECTIVE: To investigate correlations of serum miR-146a with tumor necrosis factor α(TNF-α) and T-regulatory cells(Treg) in patients with non-small cell lung cancer(NSCLC) and prognostic valuesa in the patients. METHODS: From June 2020 to November 2021, a total of 162 patients with advanced NSCLC who were treated in Cangzhou Central Hospital were selected for our study. Levels of miR-146a in patients were detected using fluorescence quantitative PCR ELISA, serum levels of miR-146a and TNF-α were detected using enzyme-linked immunoassay,and Treg levels were detected using flow cytometry.Pearson correlation was used to analyze correlations among miR-146a expressions, TNF-α concentrations and Treg proportion. Multi-factor logistic regression was used to analyze whether the relative expression levels of miR-146a was a prognostic risk factor for the patients. ROC curve was used to compare the predictive efficacy of miR-146a on prognosis. RESULTS: The relative expression levels of miR-146a was positively correlated with the concentrations of TNF-α(r=0.651, P<0.01), and negatively correlated with the proportions of Treg(r=-0.676, P<0.01). The relative expression levels of miR-146a and the concentrations of TNF-α were prognostic risk factors(OR=1.95 and 1.39,respectively;P<0.01). Treg was a prognostic protective factor for death in NSCLC patients(OR=0.53;P<0.01). The AUC of miR-146a was 0.959 and 95%CI(0.934,0.985),P<0.01. CONCLUSION: Serum miR-146a in NSCLC patients was positively correlated with TNF-α and negatively correlated with Treg,which was shown to be a risk factor for death in NSCLC patients and to have high prognostic value for NSCLC.更多还原