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不同氮源对竹屑发酵及大球盖菇菌丝生长的影响研究

Study on the Effects of Different Nitrogen Sources on the Fermentation of Bamboo Sawdust and the Mycelia Growth of Stropharia rugosoannulata

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【作者】 吴一凡; 钟楚梦; 柳丽娜; 陈胜; 鲁勇波; 谢锦忠;

【Author】 WU Yi-fan;ZHONG Chu-meng;LIU Li-na;CHEN Shen;LU Yong-bo;XIE Jin-zhong;Research Institute of Subtropical Forestry, Chinese Academy of Forestry;Anji Forestry Technology Promotion Center;Ziyunge Ecological Farm of Yuyao City;

【通讯作者】 谢锦忠;

【机构】 中国林业科学研究院亚热带林业研究所; 安吉县林业技术推广中心; 余姚市紫云阁生态农庄;

【摘要】 为探究竹屑发酵栽培大球盖菇的最佳氮源,通过以纯竹屑作对照,设置尿素、麦麸、玉米粉和菜籽饼4种不同氮源添加试验。分析了竹屑添加不同氮源的发酵温度、发酵后的理化性质及对大球盖菇菌丝生长的影响。结果表明,在研究范围内大球盖菇菌丝生长与全氮、水解氮、可溶性糖、持水孔隙度呈极显著正相关(P<0.01);与pH、碳氮比和气水比呈显著负相关(P<0.05);与EC、有机碳、磷、钾、容重和通气孔隙度呈弱相关(P>0.05)。竹屑添加4%的菜籽饼,在发酵过程中有最长的有效高温(>50℃,14 d),发酵后物料的持水性、全氮、水解性氮和可溶性糖含量显著高于其他处理,C/N为44.02,接种大球盖菇后菌丝长势最佳,日生长速度最快达3.24 mm·d-1,可作大球盖菇栽培基质使用。

【Abstract】 In order to explore the best nitrogen source for the cultivation of Stropharia rugosoannulata by bamboo sawdust fermentation, four different nitrogen sources namely urea, wheat bran, corn flour and rapeseed cake were added by taking pure bamboo chip as the control. The effects of bamboo chips added with different nitrogen sources on fermentation temperature, physical and chemical properties during the fermentation and mycelial growth of S. rugosoannulata were analyzed. The results showed that the mycelial growth of S. rugosoannulata was significantly positively correlated with total nitrogen, hydrolyzed nitrogen, soluble sugar and water holding porosity(P<0.01). It was significantly negatively correlated with pH, carbon nitrogen ratio and gas water ratio(P<0.05).It was weakly correlated with EC, organic carbon, phosphorus, potassium, bulk density and aeration porosity(P>0.05). When bamboo chips were added with 4% rapeseed cake and had a C/N ratio of 44.02, it had the longest effective temperature(>50 ℃, 14 days). The water holding capacity, total nitrogen, hydrolytic nitrogen and soluble sugar content of the fermented material were significantly higher than those of other treatments. After inoculation, the mycelium grows best and grows fastest(3.24 mm·d-1), which can be used as the cultivation substrate of S. rugosoannulata.

【关键词】 竹屑; 氮源; 发酵; 大球盖菇;
【Key words】 Bamboo sawdust; Nitrogen sources; Fermentation; Stropharia rugosoannulata;
【基金】 浙江省省院合作林业科技项目(2020SY15);宁波市科技计划项目(2019B10020);宁波市科技计划项目(2019C10048);安吉县绿色竹产业创新服务综合体重点研发项目(2022年);余姚市农业农村和社会发展科技项目(20191YYS030019)
  • 【文献出处】 竹子学报 ,Journal of Bamboo Research , 编辑部邮箱 ,2022年04期
  • 【分类号】S646
  • 【下载频次】22
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