【摘要】 目的 分析蓬子菜总黄酮对动脉粥样硬化模型家兔主动脉组织中Toll样受体（TLR）/核转录因子-κB（NF-κB）通路的影响。方法 选取75只家兔，其中15只作为正常组，其余家兔建立动脉粥样硬化模型，建模成功后随机分为模型组、低剂量组、中剂量组、高剂量组，每组15只。低剂量组、中剂量组、高剂量组分别给予0.05 mg/kg、1.00 mg/kg、1.50 mg/kg蓬子菜总黄酮灌胃，模型组和正常组给予等体积生理盐水灌胃。采用酶联免疫吸附法检测白细胞介素-6（IL-6）、白细胞介素-1α（IL-1α）、肿瘤坏死因子-α（TNF-α）、三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）;采用放射免疫法检测6-酮-前列腺素(6-keto-PGF_1α)、血栓素B₂（TXB₂）;采用蛋白质印迹法（Western Blot）检测TLR/NF-κB通路蛋白表达量。结果 高剂量组、中剂量组、低剂量组、模型组IL-6、IL-1α、TNF-α水平均高于正常组（P<0.05）;高剂量组、中剂量组、低剂量组IL-6、IL-1α、TNF-α水平低于模型组（P<0.05）;高剂量组、中剂量组IL-6、IL-1α、TNF-α水平低于低剂量组（P<0.05）;高剂量组IL-6、IL-1α、TNF-α水平低于中剂量组（P<0.05）。中剂量组、低剂量组、模型组6-keto-PGF_1α水平均低于正常组，TXB₂水平均高于正常组（P<0.05）;高剂量组、中剂量组、低剂量组6-keto-PGF_1α水平高于模型组，TXB₂水平低于模型组（P<0.05）;高剂量组、中剂量组6-keto-PGF_1α水平高于低剂量组，TXB₂水平低于低剂量组（P<0.05）;高剂量组6-keto-PGF_1α水平高于中剂量组，TXB₂水平低于中剂量组（P<0.05）。中剂量组、低剂量组、模型组TG、TC、LDL-C水平均高于正常组，HDL-C水平均低于正常组（P<0.05）;高剂量组、中剂量组、低剂量组TG、TC、LDL-C水平低于模型组，HDL-C水平高于模型组（P<0.05）;高剂量组、中剂量组TG、TC、LDL-C水平低于低剂量组，HDL-C水平高于低剂量组（P<0.05）;高剂量组TG、TC、LDL-C水平低于中剂量组，HDL-C水平高于中剂量组（P<0.05）。中剂量组、低剂量组、模型组TLR3、NF-κB p62水平高于正常组（P<0.05）;中剂量组、低剂量组TLR3、NF-κB p62水平低于模型组（P<0.05）;高剂量组、中剂量组TLR3、NF-κB p62水平低于低剂量组（P<0.05）。结论 蓬子菜总黄酮可能通过调控TLR/NF-κB信号通路中相关重要信号传导分子的蛋白表达，发挥改善动脉粥样硬化局部炎症的作用，且表现为剂量依赖。更多还原

【Abstract】 Objective To analyze the effect of flavone of Galium verum L（FGVL） on Toll-like receptors（TLR）/nuclear factor-κB（NF-κB） pathway in aortic tissue of rabbits with atherosclerosis.Methods Seventy-five rabbits were selected, of which 15 were used as the normal group, and the rest of the rabbits were established atherosclerosis models.After successful modeling, the rabbits were randomly divided into model group, low-dose group, medium-dose group, and high-dose group, with 15 rabbits in each group.The rabbits were given 0.05 mg/kg, 1.00 mg/kg, and 1.50 mg/kg of FGVL intragastric administration, respectively.The rabbits in model group and normal group were given equal volume saline intragastric administration.The levels of interleukin-6（IL-6）,interleukin-1α（IL-1α）,tumor necrosis factor-α（TNF-α）,triglyceride（TG）,total cholesterol（TC）,low-density lipoprotein cholesterol（LDL-C）,and high-density lipoprotein cholesterol（HDL-C） were detected by enzyme-linked immunosorbent assay（ELISA）.Radioimmunoassay was used to detect 6-keto-prostaglandin F_1α(6-keto-PGF_1α) and thromboxane B₂（TXB₂）.The protein expression of TLR/NF-κB pathway was detected by Western Blot method.Results The levels of IL-6,IL-1α,and TNF-α in high-dose group, medium-dose group, low-dose group, and model group were higher than those in normal group（P<0.05）,which in high-dose, medium-dose, and low-dose groups were lower than those in model group, with a dose-dependent manner（P<0.05）.The levels of 6-keto-PGF_1α in medium-dose group, low-dose group, and model group were lower than that in normal group, and the level of TXB₂ was higher than that in normal group（P<0.05）.The levels of 6-keto-PGF_1α in high-dose, medium-dose, and low-dose groups were higher than that in model group, while the levels of TXB₂ were lower than that in model group, with a dose-dependent manner（P<0.05）.The levels of TG,TC,and LDL-C in medium-dose group, low-dose group, and model group were higher than those in normal group, while the HDL-C levels were lower than that in normal group（P<0.05）.The levels of TG,TC,and LDL-C in high-dose, medium-dose, and low-dose groups were lower than those in model group, while the HDL-C levels were higher than that in model group, and with a dose-dependent manner（P<0.05）.The levels of TLR3 and NF-κB p62 in medium-dose group, low-dose group, and model group were higher than those in normal group（P<0.05）,which in high-dose, medium-dose, and low-dose groups were lower than those in model group（P<0.05）,and which in high-dose and medium-dose groups were lower than those in low-dose group（P<0.05）.Conclusion FGVL may play a role in improving local inflammation of atherosclerosis, with a dose dependent manner by regulating the protein expression of important signal transduction molecules in TLR/NF-κB signaling pathway.更多还原