【摘要】 目的 观察清肺排毒汤对人类冠状病毒229E株感染Huh7细胞后细胞因子风暴相关炎症因子的影响。方法 用人类冠状病毒229E株感染Huh7细胞，将清肺排毒汤作用细胞72 h后收集培养上清和细胞样本，分别用于检测细胞因子风暴相关炎症因子和病毒载量。结果 清肺排毒汤对Huh7细胞及培养上清中病毒RNA无明显抑制作用（均为P>0.05）；可使感染了229E冠状病毒的Huh7细胞分泌的肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、干扰素γ(IFN-γ)、趋化因子配体2(CCL2)、趋化因子配体3(CCL3)、趋化因子配体5(CCL5)、干扰素诱导蛋白10(IP-10)mRNA的表达降低（P<0.05,P<0.01），但对CCL2、CCL3、CCL5 mRNA的抑制效果较弱；对细胞培养上清液中的TNF-α、IL-6、IL-1β、IFN-γ蛋白水平均有抑制作用（P<0.05,P<0.01）。结论 清肺排毒汤可能不是主要通过抑制炎细胞趋化因子和病毒复制起作用，而是主要通过抑制细胞因子风暴相关的炎症因子起作用。更多还原

【Abstract】 Objective To observe the effects of Qingfei Paidu Decoction(QFPDD)on inflammatory cytokines,which were correlated with the cytokine storm after human coronavirus 229E(HCoV-229E) infection of Huh7 cells.Methods Huh7 cells were infected with HCoV-229E. Then QFPDD was added in Huh7 cell culture system. After72 hours of coculture,the culture supernatant and cells were collected for measuring the levels of viral load and inflammatory cytokines associated with cytokine storm. Results There were no obvious inhibition effect of QFPDD on viral RNA in supernatant and cells was observed(P>0.05). QFPDD treatment remarkably decreased the mRNA expression in HCoV-229E-infected Huh7 cells,including TNF-α,IL-6,IL-1β,IFN-γ,CCL2,CCL3,CCL5and IP-10(P<0.05,P<0.01). However,the reduction of CCL2,CCL3,CCL5 mRNA levels was found only at high concentrations of QFPDD. QFPDD has significant inhibitory effect on TNF-α,IL-6,IL-1β,IFN-γ protein levels in the culture supernatant(P<0.05, P<0.01). Conclusion QFPDD may not work by suppressing inflammatory chemokines and virus replication, but it may work primarily by suppressing inflammatory cytokines associated with cytokine storm.更多还原