【摘要】 目的 研究不同剂量的黄芪多糖（Astragalus Polysacharin,APS）对糖尿病溃疡炎症期相关因素的影响。方法 将36只大鼠随机分为正常组、糖尿病溃疡模型组、生长因子对照组和黄芪多糖低(100 mg·kg^-1)、中(200 mg·kg^-1)、高(400 mg·kg^-1)剂量组，通过腹腔注射链脲佐菌素（Streptozocin,STZ）和外科方法建立糖尿病大鼠溃疡模型。各组于创面模型复制后当日开始给药，换药前均以1∶5 000呋喃西林溶液清洁创面。正常组、糖尿病溃疡模型组，以生理盐水灌胃，外敷生理盐水纱布；生长因子对照组，以生理盐水灌胃，外用碱性成纤维细胞生长因子；各黄芪多糖组外敷生理盐水纱布，不同剂量黄芪多糖灌胃。术后3 d取创面肉芽组织为所需实验样品，分别运用免疫荧光和免疫组化方法检测M1、M2型巨噬细胞、白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、白细胞介素10（IL-10）、肿瘤坏死因子α（TNF-α）的水平。结果 炎症期糖尿病溃疡模型组创面M1型巨噬细胞数量明显较少，IL-1β、IL-6、TNF-α、IL-10的表达水平都明显低于正常组（P<0.01）；黄芪多糖中、高剂量组M1型巨噬细胞表达较多；黄芪多糖中、高剂量组和生长因子组创面IL-1β、IL-6、TNF-α的表达水平明显高于糖尿病溃疡模型组（P<0.05,P<0.01）；黄芪多糖中剂量组IL-1β、IL-6、TNF-α水平明显高于黄芪多糖低剂量组（P<0.01），黄芪多糖高剂量组IL-10的表达水平高于糖尿病溃疡模型组（P<0.05）。结论 黄芪多糖能在糖尿病溃疡大鼠炎症期促进M1型巨噬细胞分泌，增加促炎因子、抑炎因子的表达，维护创面正常炎症反应的进行。更多还原

【Abstract】 Objective To study the effects of different doses of Astragalus polysaccharides（APS） on the factors related to inflammation stage of diabetic ulcers.Methods Thirty-six rats were randomly divided into normal group,diabetic ulcer model group,growth factor control group,APS low-dose(100 mg·kg^-1)group,APS medium-dose(200 mg·kg^-1) group, and APS high-dose(400 mg·kg^-1) group. The diabetic ulcer model was established by intraperitoneal injecting streptozotocin（STZ）and surgical methods. Each group was administered on the day of wound model replication and cleaned the wound with 1∶5 000 furacillin solution before medication. Normal group and diabetic ulcer model group were given normal saline,the wound was covered by gauze soaked with normal saline.Growth factor control group was also given normal saline,the wound was treated with the fibroblast growth factor.APS groups were given different doses of APS and the wound was covered by gauze soaked with normal saline. Three days after operation,the granulation tissue of wounds was taken as the experimental sample,and the levels of M1 and M2 macrophages,IL-1β,IL-6,IL-10 and TNF-α were detected by immunofluorescence and immunohistochemical methods.Results The number of M1-type macrophages in the model group was significantly lower than that in the normal group,and the levels of IL-1β,IL-6,TNF-α and IL-10 were significantly lower than those in the normal group（P<0.01）. M1-type macrophages were strongly expressed in the medium-and high-dose APS groups. The levels of IL-1β,IL-6 and TNF-α of the medium-and high-dose APS groups and the growth factor group were significantly higher than those of the diabetes ulcer model group（P<0.05,P<0.01）. The levels of IL-1β,IL-6and TNF-α in the medium-dose APS group were significantly higher than those in the low-dose APS group（P<0.01）,and the expression of IL-10 in the high-dose APS group was significantly higher than that in the diabetic ulcer model group（P<0.05）.Conclusion APS can promote the secretion of M1-type macrophages, increase the expression of pro-inflammatory factors and anti-inflammatory factors, and maintain the normal inflammatory response of the wound in diabetic ulcer rats during the inflammatory stage.更多还原