【摘要】 目的 通过基于质谱分析技术寻找寻常型银屑病患者尿液潜在生物标记物。方法 应用液相色谱-气相色谱-质谱联用技术（LC-GC-MS）技术对7例无家族史的寻常型银屑病女性患者和7例有家族史的寻常型银屑病女性患者及14例健康志愿者血浆进行检测，分析数据采取聚类法（CA）、主成分分析法（PCA）、偏最小二乘判别分析法（PLS-DA）和t检验等统计学方法进行分析，寻找寻常型银屑病患者与健康者尿液差异性代谢物。结果 寻常型银屑病女性患者尿液标本中L-丙氨酸、天冬氨酸较正常对照组升高，甘油（丙三醇）水平与正常对照组相比降低。结论寻常型银屑病患者不仅存在糖代谢及氨基酸代谢的紊乱，还可能合并肝肾代谢功能障碍。该结果可能对深入研究银屑病的发病机制，同时为开发新的治疗靶点及预防银屑病的发生、评估银屑病的预后提供新的思路。更多还原

【Abstract】 Objective In order to detect the potential plasma biomarkers for patients with vulgaris psoriasis by technology based on liquid chromatography-gas chromatography-mass spectrometry(LC-GC-MS) technique. Methods LC-GC-MS technique was applied to examine urine samples from seven female patients without psoriasis family history, seven female patients had positive psoriasis family history and 14 health female volunteers. The data were analyzed using cluster analysis(CA),principal component analysis(PCA),partial least square-discriminant analysis(PLS-DA) and t-test. The differential metabolites in urine between female patients with psoriasis vulgaris and those in healthy female volunteers were analyzed. Results Compared with healthy volunteers,the levels of L-alanine,aspartic acid were increased;the level of glycerol was decreased. Conclusion This study suggests that patients with psoriasis vulgaris have glucose metabolism and amino acid metabolism,may accompanied liver and kidney functional damage. The study aims to provide a new thought on the protection of psoriasis incidence,evaluating the prognosis of psoriasis,developing new therapeutic target,and laying the foundation of psoriasis therapy.更多还原