【摘要】 为探究二氧化碳（CO₂）在华北大黑鳃金龟Holotrichia oblita幼虫定位寄主植物根系时所发挥的作用,对其开展了触角电位生理反应及行为生测试验。研究结果表明,CO₂浓度为0.75%、1.5%、3%、6%时均能显著激发幼虫的触角电位和趋性反应,且随着浓度增加幼虫对CO₂的趋向爬行所用时间显著缩短;根系分泌物同样能引起华北大黑鳃金龟幼虫的触角电位和趋性行为反应,且与1.5%CO₂间无显著差异;将1.5%CO₂分别与棕榈酸甘油酯、2-丁烯酸、肉豆蔻酸、十四烷、4-甲基-2-氧戊酸、十二烷、环己六醇、邻二甲苯和对羟基苯甲酸等9种根系分泌物混合后对试虫引诱效果增强,其趋向爬行时间显著缩短。以上结果显示,CO₂在华北大黑鳃金龟幼虫定位寄主植物根系过程中发挥了重要的指引和增效作用,试验结果可为华北大黑鳃金龟幼虫的绿色防治提供新思路。更多还原

【Abstract】 In order to explore the role of carbon dioxide（CO₂） in locating the roots of host plants by the larvae of Holotrichia oblita, the electroantennogram（EAG） and behavioral responses were assayed. The results showed that the stimulation of 0.75%, 1.5%, 3%, 6% CO₂ elicited a strong reaction of the larvae, and their crawling speed became faster with increasing concentration of CO₂. The root exudates could also induce an EAG and chemotaxis response of H.oblita larvae, and the results were similar to that of the group of 1.5% CO₂. Furthermore, mixing 1.5% CO₂ with nine kinds of root exudates, i.e., tripalmitin, 2-butenoic acid, myristic acid, tetradecane, 4-methyl-2-oxovaleric acid, dodecane, inositol, o-xylene and p-hydroxybenzoic acid, respectively, could enhance the attraction effect and significantly shorten the crawling time. These results suggested that CO₂ played an important role in guiding and enhancing the locating capability of the larvae of H.oblita for the host plant roots, which provides a new idea for green control of H.oblita larvae.更多还原