【摘要】 本研究旨在建立基于同位素内标的液相色谱串联质谱(LC-MS/MS)分析方法，以同步检测小鼠血清中28种胆汁酸。样本用甲醇沉淀蛋白质后，采用Waters BEH C18超高效液相色谱柱(1.7μm, 2.1mm×100 mm)分离，以0.1%甲酸-水和0.1%甲酸-乙腈作为流动相进行梯度洗脱，流速0.5 mL/min,采用负离子模式进行质谱检测。结果显示：胆汁酸在定量范围内线性关系良好，检测限在0.2～1.5 ng/mL,样品中胆汁酸同位素内标的加标回收率均在83.5%～108.4%,变异系数均小于14.9%。采用该方法检测了小鼠血清样品中的各胆汁酸含量，所有样品均检测出了25种胆汁酸。结果表明，本研究所建立的LC-MS/MS法操作简捷，灵敏度高，多靶标同步分析能力强，能够用于小鼠血清胆汁酸谱的检测。更多还原

【Abstract】 A liquid chromatography-tandem mass spectrometry(LC-MS/MS) method based on isotope internal standard was established for simultaneous determination of 28 bile acids in mouse serum samples. Samples were extracted with methanol and separated on a Waters BEH C18 ULTRA-high performance liquid chromatography(1.7 μm, 2.1 mm×100 mm) column. Gradient elution was performed with 0.1% formic acid-water and 0.1% formic acid-acetonitrile as mobile phases at the flow rate of 0.5 mL/min. Negative ion mode was used for mass spectrometry. The results showed that the calibration curves of 28 bile acids were linear in the quantitative range, the detection limit was 0.2-1.5 ng/mL. The average recovery ratios of bile acid isotopes ranged from 83.5% to 108.4%,and the coefficients of variation were all less than 14.9%. The contents of bile acids in mouse serum samples were determined by this method, and 25 bile acids were detected in all mouse serum samples. Thus, the LC-MS/MS method established in this study has the advantages of simple operation, high sensitivity and strong multiplexing detection capability, and facilitates the detection of serum bile acid profile in mice.更多还原