【摘要】 【目的】：研究CD4 T淋巴细胞内的基础ATP浓度代替CD4/CD8比值用于评估结核病感染者免疫状态的可行性。【方法】：本研究通过磁珠法分离相同体积全血中的CD4、CD8细胞，并计算CD4和CD8细胞的基础ATP浓度来评估结核病感染者的免疫状态，以QFM（Quantiferon Monitor）检测法作为对照。【结果】：研究发现传统的CD4/CD8比值或CD4 T细胞计数方法不适用于结核病患者。采用CD4细胞的基础ATP浓度来监测活动性结核患者和健康人群，在以290.7×10^-6g/L为cut-off值，AUROC为0.8719，其灵敏度为86.21%，特异性为76.19%，差异有统计学意义（P<0.000 1）。【结论】：本研究首次使用非外界刺激条件下CD4+T淋巴细胞胞内基础ATP浓度来评估结核病感染者的免疫状态。提示CD4+T淋巴细胞的基础ATP浓度比CD4/CD8比值可以更好用于评估结核病患者的免疫状态。更多还原

【Abstract】 【Objective】To investigate the feasibility of basal ATP concentration in CD4 T lymphocytes instead of CD4/CD8 ratio in assessing the immune status of tuberculosis-infected patients.【Methods】In this study,the CD4 and CD8cells in the same volume of whole blood were separated by magnetic beads method,and the basal ATP concentration of CD4 and CD8 cells was calculated to evaluate the immune status of tuberculosis-infected patients. Quantiferon Monitor（QFM）assay was used as a control.【Results】Our study confirmed that traditional CD4/CD8 ratio andCD4 T cell counting methodswerenot suitable for TB patients. Using the basal ATP concentration of CD4 cells to monitor active tuberculosis patients and healthy people,when the cut-off value was 290.7×10^-6g/L,the sensitivity was 86.21%,and the specificity was 76.19%,and the difference was statistically significant（P<0.000 1）.【Conclussions】In this study,to the best of our knowledge,we have for the first time used the basal ATP concentration in CD4+T lymphocytes to evaluate the immune status of tuberculosis-infected patients under non-external stimulation conditions. It is suggested that the basal ATP concentration of CD4+T lymphocytes can be better used to evaluate the immune status of tuberculosis patients than CD4/CD8 ratio.更多还原