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4株香菇菌株菌丝在不同母种培养基上的生长特性比较

Comparison of Growth Characteristics of Mycelium of Four Lentinula edodes Strains on Different Stock Culture Mediums

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【作者】 朱金霞; 冯锐; 任怡莲; 郑国保; 孔德杰; 李苗;

【Author】 ZHU Jin-xia;FENG Rui;REN Yi-lian;ZHENG Guo-bao;KONG De-jie;LI Miao;Institute of Forestry Sciences Agricultural Biotechnology Research Center,Ningxia Academy of Agriculture and Forestry Science;Ningxia Key Laboratory of Agricultural Biotechnology;Institutue of Agricultural Economy and Information Technology,Ningxia Academy of Agriculture and Forestry Sciences;Ningxia Academy of Agriculture and Forestry Sciences;

【通讯作者】 冯锐;任怡莲;

【机构】 宁夏农林科学院农业生物技术研究中心; 宁夏农业生物技术重点实验室; 宁夏农林科学院农业经济与信息技术研究所; 宁夏农林科学院;

【摘要】 以香菇菌株“818”“申香215”“申香1504”和“向阳2号”为研究对象,比较其在不同母种培养基上的菌丝生长特性及菌落生长速率,筛选出各菌株的最适母种培养基。结果表明,4个香菇菌株在不同培养基上菌丝体颜色、密度、均匀度均存在一定差异。改良PDA培养基仍是香菇菌株“818”和“向阳2号”的最佳母种培养基,添加枸杞枝条提取物在一定程度上可促进菌株“申香215”菌丝生长,但效果不显著(P>0.05)。玉米芯提取物、枸杞枝条提取物和红枣枣核提取物可使“申香1504”菌丝生长速率分别提高6.68%、5.32%和0.95%。另筛选出各香菇菌株最适母种培养基1个。研究结果可为进一步提高香菇母种质量提供技术支撑。

【Abstract】 Taking strain‘818’‘Shenxiang 215’‘Shenxiang 1504’‘Xiangyang 2’of Lentinula edodes as the subject, the growth characteristics of mycelium and growth rates of colonies on different stock culture mediums were compared. The results showed that there were some differences in color, density and unifomity on different media. The improved PDA medium was still the best medium for L. edodes strain‘818’and‘Xiangyang 2’, addition the extraction of the branch of Lycium barbarum could promote the growth of mycelium of ‘Shenxiang 215’, but the effect was not significant(P >0.05), the rate of the growth of mycelium of ‘Shenxiang 1504’increased by 6.68%, 5.32% and 0.95% by addition the extraction of corncob, the branch of L. edodes. and jujube nucleus, respectively, and one stock culture medium were selected for 4 varieties of L. edodes. respectively.The results can provide technical support for further improving the quality of stock culture of L. edodes.

【关键词】 香菇; 菌丝; 生长特性; 生长速率;
【Key words】 Lentinula edodes; mycelium; growth characteristics; growth rate;
【基金】 宁夏回族自治区重点研发计划项目(2020BBF03006);宁夏农林科学院农业自主科技创新对外科技合作专项(DWX-2018026、DWX-2020007)
  • 【文献出处】 中国食用菌 ,Edible Fungi of China , 编辑部邮箱 ,2022年05期
  • 【分类号】S646.12
  • 【下载频次】159
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