【摘要】 采用基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)和电喷雾质谱(ESI-MS)研究含巯基的生命物质(氨基酸、肽及蛋白质)与MALDI常用基质α-氰基-4-羟基肉桂酸(HCCA)之间的加成反应，并分别考察了其在酸性、弱酸性和碱性条件下的反应情况。结果表明，在碱性条件下，含巯基的生命物质易与HCCA发生加成反应，使待测物在质谱图中产生189.0 u的质量数迁移。此外，使用高分辨质谱分析加成产物，并通过串联质谱分析进一步确认其为共价结合产物。此研究为确定MALDI基质与含巯基的生命物质之间的加成反应提供了依据，以期减少质谱中基质对含有巯基化合物测试的干扰，为基质的选择提供参考。更多还原

【Abstract】 The addition reactions between the common matrix α-cyano-4-hydroxy cinnamic acid(HCCA) in matrix assisted laser desorption(MALDI) and the sulfhydryl-contained compounds were investigated using matrix assisted laser desorption time of flight mass spectrometry(MALDI-TOF MS) and electrospray mass spectrometry(ESI-MS), and the corresponding reactivity was also compared in the acid, weak acid and alkaline conditions, respectively. The results showed that the sulfhydryl-contained compounds could much easily undergo addition reaction with HCCA under alkaline conditions, resulting in a mass shift of 189.0 u in the mass spectrum. In addition, the identity of the reaction product was supported by high resolution mass spectrometry analysis, and was further identified as covalent bonding products by tandem MS analysis. This work provides a proof for the addition reaction between MALDI matrix and sulfhydryl-contained compounds, so as to reduce the interference of the matrix in the MALDI-TOF MS analysis of the relevant compounds, and to provide a reference for the selection of matrix.更多还原