【摘要】 目的通过高效液相色谱(HPLC)特征图谱定性分析和指标成分定量分析结合模式识别,建立沙棘银花颗粒提取工艺综合评价方法。方法以提取溶剂种类、用量和提取时间为考察因素,对各工艺水平所得提取物进行HPLC特征图谱定性分析、相似度评价、聚类分析和指标成分含量测定。结果建立沙棘银花颗粒提取工艺的HPLC特征图谱共有模式,确定20个共有峰,7种提取工艺相似度在0.809～0.999,聚类分析法将7种工艺分为两类。维采宁-2、芹糖甘草苷、异绿原酸B和槲皮素4种指标成分定量分析表明,工艺S4(提取溶剂为12倍量水,提取时间为2 h)有相对高的成分转移率。结论建立的HPLC定性定量分析结合模式识别的方法为中药制备工艺研究提供了一种综合评价方式。更多还原

【Abstract】 Objective To establish HPLC characteristic chromatogram and quantitatively analyse the index components combined with pattern recognition,and to comprehensively evaluate the extraction technology of Shaji Yinhua granules.Methods The extraction solvent category,volume,and extraction time were measured.HPLC characteristic chromatogram qualitative analysis,similarity evaluation,clustering analysis and index component analysis were conducted to distinguish the extraction processes.Results The common pattern of HPLC characteristic chromatogram for the extraction of Shaji Yinhua granules was established and 20 common peaks were identified.The 7 extraction processes were calssified into two groups by cluster analysis and their similarity ranged from 0.809 to 0.999.The quantitative analysis of 4 index components (vicenin-2,liquiritinapioside,isochlorogenic acid B and quercetin) showed that process S4 (12 times water as the extraction solvent,extraction for 2 h) had a relatively high component extraction ratio.Conclusion The established HPLC qualitative and quantitative analysis and pattern recognition model provide a comprehensive evaluation of the study on preparation of traditional Chinese medicine.更多还原