【摘要】 利用常压室温等离子体（ARTP）诱变方法对实验室保藏的酿酒酵母（Saccharomyces cerevisiae）SC-62进行诱变，通过试验确定最佳诱变条件为处理时长80 s，此条件下菌株SC-62致死率84%。将诱变获得的菌株进行初筛、复筛和发酵性能测定。结果显示，筛选出一株耐酸性强、发酵性能优良的正突变菌株A-107，其在p H为2.5的发酵培养基上培养6 d后测得的发酵力[6.21 g CO2/（100 m L·24 h）]和酒精产量（11.52%vol）较出发菌株SC-62分别提高了37%和30%，突变菌株A-107可耐受16%乙醇、100 g/L NaCl、500 g/L葡萄糖，耐受性和遗传稳定性良好。更多还原

【Abstract】 Saccharomyces cerevisiae SC-62 was mutated by atmospheric and room temperature plasma(ARTP). The optimal conditions were determined as treatment time 80 s, and the fatality rate of the strain SC-62 was 84%. The strains obtained by mutagenesis were tested for primary screening,rescreening, and fermentation performance. The results showed that a positive mutant strain A-107 with strong acid resistance and good fermentation performance was screened. The fermentation capacity [6.21 g CO2/(100 ml·24 h)] and alcohol yield(11.52%vol) increased by 37% and 30% than the original strain SC-62 after 6 d of culture on pH 2.5 fermentation medium, respectively. The mutant strain A-107 could tolerate 16% ethanol, 100 g/L NaCl and 500 g/L glucose, the tolerance and genetic stability were good.更多还原