【摘要】 目的 探讨五味子根、叶多糖对小鼠镇静催眠及抗焦虑的作用.方法 取清洁级ICR雄性小鼠随机分为4组，即空白对照组、根多糖组、叶多糖组、地西泮组，每组30只.应用小鼠自主活动实验观察镇静效果，应用高架十字迷宫实验观察抗焦虑作用，应用戊巴比妥钠协同睡眠实验观察催眠作用，应用酶联免疫吸附法检测脑组织中γ-氨基丁酸(γ-aminobutyric acid, GABA)、谷氨酸(glutamate, Glu)含量.结果 与空白对照组比较，根多糖组小鼠站立、活动次数显著减少(P<0.01),叶多糖组小鼠活动次数减少(P<0.05);根多糖组和叶多糖组开臂次数/在臂次数比(OE%)和开臂滞留时间/在臂滞留时间比(OT%)增加(P<0.01);在戊巴比妥钠诱导小鼠睡眠的实验中，与空白对照组比较，阈下剂量时根多糖及叶多糖组小鼠睡眠只数均增加，阈剂量时根多糖组小鼠睡眠潜伏期减少，睡眠时间显著延长(P<0.01),叶多糖组小鼠睡眠时间显著延长(P<0.01);与空白对照组比较，根多糖组显著增加小鼠脑组织中GABA含量(P<0.01),根多糖和叶多糖组均显著降低Glu含量(P<0.01),显著增加GABA/Glu(P<0.05或P<0.01).结论 北五味子的根、叶多糖具有镇静催眠及抗焦虑作用.更多还原

【Abstract】 Objective To study the sedative and hypnotic effects of polysaccharides from the roots and leaves of Schisandra chinensis in mice.Method Clean-grade ICR male mice were randomly divided into 4 groups with 30 mice in each group, blank control group, root polysaccharide group, leaf polysaccharide group and diazepam group.The autonomous activities of mice were used to observe the sedative effects; The elevated plus mase was used to observe the anti-anxiety effects; The subthreshold and threshold dose synergistic sleep test of pentobarbital sodium was used to observe the hypnotic effect.Enzyme-linked immunosorbent assay(ELISA) was used to detect the content of γ-aminobutyric acid(GABA) and glutamate(Glu) in mouse brain tissue.Results Compared with the blank control group, the number of standing and spontaneous activities of the mice significantly reduced in root polysaccharide group(P<0.01);The number of spontaneous activities of the mice in leaf polysaccharide group decreased(P<0.05);The percentages of the retention time at open arm/close arm and the entering time to open arm/close arm increased in both root and leaf polysaccharide groups(P<0.01).Under the subthreshold dose of pentobarbital sodium, the number of sleep mice increased in both root and leaf polysaccharide groups; Under the threshold dose of pentobarbital sodium, the sleep latency was shortened and sleep time increased in mice in root polysaccharide group(P<0.01);Sleep time of mice was significantly prolonged in leaf polysaccharide group(P<0.01);The content of GABA in brain tissue of mice in root polysaccharides group significantly increased(P<0.01),while Glu content significantly decreased(P<0.01) and GABA/Glu ratio significantly increased in root polysaccharides and leaf polysaccharides groups(P<0.05 or P<0.01).Conclusion Polysaccharides from root and leaf of Schisandra chinensis have sedative, hypnotic and anti-anxiety effects.更多还原