【摘要】 目的 研究长链非编码RNA(lncRNA)LINC00958影响乳腺癌MDA-MB-231细胞增殖、迁移、侵袭和凋亡的作用与机制。方法 定量逆转录聚合酶链反应(qRT-PCR)检测LINC00958和miR-597-5p在乳腺癌组织中的表达。MDA-MB-231细胞转染si-LINC00958或miR-597-5p或共转染si-LINC00958和anti-miR-597-5p。qRT-PCR检测LINC00958和miR-597-5p表达，四甲基偶氮唑蓝(MTT)检测MDA-MB-231细胞增殖，Transwell检测细胞迁移、侵袭，流式细胞术检测细胞凋亡，Western印迹检测Ki-67、基质金属蛋白酶(MMP)-2、MMP-9、B细胞淋巴瘤(Bcl)-2、Bcl-2相关X蛋白(Bax)表达。双荧光素酶报告实验分析LINC00958与miR-597-5p的靶向关系。结果 与癌旁组织比较，41例乳腺癌组织中LINC00958表达显著上调，miR-597-5p表达显著下调(P<0.05)。抑制LINC00958表达显著降低MDA-MB-231细胞增殖、Ki-67表达水平、迁移、侵袭细胞数、MMP-2、MMP-9、Bcl-2表达水平，显著提高细胞凋亡率、Bax表达水平(P<0.05),与miR-597-5p过表达的结果相同。LINC00958靶向调控miR-597-5p的表达。干扰miR-597-5p表达后，抑制LINC00958表达对乳腺癌MDA-MB-231细胞增殖、迁移、侵袭和凋亡的作用被显著逆转(P<0.05)。结论 LINC00958通过靶向下调miR-597-5p,增强乳腺癌MDA-MB-231细胞的增殖、迁移、侵袭，并减轻细胞凋亡。更多还原

【Abstract】 Objective To study the effect and mechanism of long non-coding RNA(lncRNA) LINC00958 on the proliferation, migration, invasion and apoptosis of breast cancer MDA-MB-231 cells.Methods Quantitative reverse transcription polymerase chain reaction(qRT-PCR) was used to detect the expression of LINC00958 and miR-597-5 p in breast cancer tissues. MDA-MB-231 cells were transfected with si-LINC00958 or miR-597-5 p, or co-transfected with si-LINC00958 and anti-miR-597-5 p. qRT-PCR was used to detect the expression of LINC00958 and miR-597-5 p, tetramethyl azolium blue(MTT) was adopted to detect the proliferation of MDA-MB-231 cells, Transwell was used to detect cell migration and invasion, flow cytometry was used to detect cell apoptosis, Western blot was adopted to detect Ki-67 and matrix metalloproteinases(MMP)-2, MMP-9, B-cell lymphoma(Bcl-2), Bcl-2 related X protein(Bax) expression. The dual luciferase report experiment was used to analyze the targeting relationship between LINC00958 and miR-597-5 p.Results Compared with adjacent tissues, the expression of LINC00958 was significantly up-regulated and the expression of miR-597-5 p was significantly down-regulated in 41 breast cancer tissues(P<0.05). Inhibition of LINC00958 expression significantly reduced MDA-MB-231 cell proliferation, Ki-67 expression level, migration, number of invaded cells, MMP-2, MMP-9, Bcl-2 expression levels, and significantly increased cell apoptosis rate and Bax expression level(P<0.05), which was the same as the result of miR-597-5 p overexpression. LINC00958 targeted the expression of miR-597-5 p. After interfering with the expression of miR-597-5 p, the effect of inhibiting the expression of LINC00958 on the proliferation, migration, invasion and apoptosis of breast cancer MDA-MB-231 cells was significantly reversed(P<0.05). Conclusions LINC00958 could enhance the proliferation, migration and invasion of breast cancer MDA-MB-231 cells by targeting down-regulation of miR-597-5 p, and reducing cell apoptosis.更多还原