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褐球固氮菌对干旱胁迫下小麦幼苗生长的影响

Effects of Azotobacter chroococcum on seedling growth of wheat under drought stress

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【作者】 葛红莲刘中华张福丽

【Author】 GE Honglian;LIU Zhonghua;ZHANG Fuli;College of Life Science and Agronomy, Zhoukou Normal University;

【机构】 周口师范学院生命科学与农学学院

【摘要】 为研究褐球固氮菌(Azotobacter chroococcum)AC17菌株对小麦幼苗干旱胁迫的缓解作用,采用20%聚乙二醇6000模拟干旱胁迫处理水培的小麦幼苗,研究小麦幼苗的生长和生理指标的变化。结果表明:AC17+干旱胁迫处理组小麦幼苗的株高、根长、鲜重、干重分别比干旱胁迫组提高了28.51%、25.60%、119.23%、37.50%;小麦幼苗叶绿素、可溶性糖和可溶性蛋白的含量比干旱胁迫组分别提高了50.00%、24.69%、70.39%;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性比干旱胁迫组分别提高了6.54%、25.45%、35.18%;丙二醛(MDA)含量比干旱胁迫组降低了25.11%。因此,AC17菌株能有效缓解干旱胁迫对小麦幼苗生长的抑制作用,增强小麦幼苗的抗旱能力。

【Abstract】 In order to study the relieving effect of Azotobacter chroococcum AC17 on wheat seedling under drought stress, a hydroponic experiment with wheat seedling was carried out. Wheat seedlings were subjected to 20%(w/v) polyethylene glycol(PEG-6000) to simulate the drought stress. The changes of the growth and physiological parameters of wheat seedlings under drought stress were investigated. The results showed that the shoot height, root length, and dry and fresh weights of wheat seedlings inoculated with strain AC17 under drought stress increased significantly by 28.51%, 25.60%, 119.23% and 37.50%, respectively, than those of the drought stress group plants. Compared to the drought stress group, the chlorophyll, soluble sugar and soluble protein content of wheat seedlings inoculated with strain AC17 under drought stress increased by 50.00%, 24.69% and 70.39% respectively, the activities of superoxide dismutase(SOD), catalase(CAT) and peroxidase(POD) increased by 6.54%, 25.45%, 35.18%, and the malondialdehyde(MDA) content decreased by 25.11%. Therefore, strain AC17 alleviated the inhibition of drought stress on the growth of wheat seedlings and enhanced the drought resistance of wheat seedlings.

【基金】 河南省高等学校重点科研项目“干旱胁迫下复合菌剂对小麦生长和土壤肥力的影响”(21B210013);河南省科技攻关项目(222102110060)
  • 【文献出处】 周口师范学院学报 ,Journal of Zhoukou Normal University , 编辑部邮箱 ,2022年05期
  • 【分类号】S512.1;S144
  • 【下载频次】12
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