【摘要】 目的 探讨甲状腺激素受体相互作用因子13(TRIP13)在B细胞淋巴瘤组织中的表达及其临床意义。方法 选取2018年9月至2020年11月在嘉兴市第二医院就诊后进行淋巴结活检，并留取多余的新鲜淋巴结活检组织冻存的B细胞淋巴瘤患者44例为研究对象，参照2016年WHO对B细胞淋巴瘤分型诊断标准，将44例患者分为惰性淋巴瘤组20例和侵袭性淋巴瘤组24例。采用RT-PCR法检测B细胞淋巴瘤组织中TRIP13 m RNA表达水平，比较惰性淋巴瘤组和侵袭性淋巴瘤组TRIP13m RNA表达水平。采用En Vision免疫组化染色法检测TRIP13蛋白表达情况。根据TRIP13免疫组化表达情况，将B细胞淋巴瘤患者分为TRIP13阴性组（0）和TRIP13阳性组（≥1+），比较两组患者性别、年龄、B细胞淋巴瘤分型情况、增殖指数（Ki-67）、乳酸脱氢酶、β2微球蛋白、白蛋白水平、T辅助细胞比例、活化CD4~+细胞比例、活化CD8~+细胞比例及CD4~+/CD8~+等临床特征；再根据TRIP13免疫组化表达强度，将TRIP13阳性患者分为TRIP13弱阳性组（1+）和TRIP13强阳性组（≥2~+），比较两组患者上述临床特征。结果 惰性淋巴瘤组和侵袭性淋巴瘤组TRIP13 m RNA表达水平分别为0.37(0.17,1.36)和0.69(0.28,1.15)，两组比较差异无统计学意义（P>0.05）。TRIP13阳性组患者TRIP13 m RNA表达水平、Ki-67水平及活化CD8~+细胞比例均高于TRIP13阴性组，T辅助细胞比例、活化CD4~+细胞比例、CD4~+/CD8~+均低于TRIP13阴性组，差异均有统计学意义（均P<0.05）。TRIP13弱阳性组TRIP13 m RNA表达水平、Ki-67水平、活化CD8~+细胞比例均低于TRIP13强阳性组，活化CD4~+细胞比例、CD4~+/CD8~+均高于TRIP13强阳性组，差异均有统计学意义（均P<0.05）。结论 B细胞淋巴瘤组织中TRIP13阳性及强阳性与更高的Ki-67水平、活化CD8~+细胞比例及更低的活化CD4~+细胞比例有关，提示TRIP13阳性可能和肿瘤细胞增殖及免疫调控异常等发病机制有关。更多还原

【Abstract】 Objective To investigate the expression of thyroid hormone receptor interactor 13(TRIP13) in B-cell lymphoma tissues and its relationship with clinical features. Methods Forty-four patients with B-cell lymphoma who underwent lymph node biopsy in the Second Hospital of Jiaxing from September 2018 to November 2020 were enrolled. According to the diagnostic criteria of B-cell lymphoma, there were 20 cases of indolent lymphoma and 24 cases of aggressive lymphoma. The expression of TRIP13 m RNA and protein in B-cell lymphoma tissues was detected by RT-PCR and En Vision immunohistochemical staining, respectively. According to the immunohistochemical expression of TRIP13, B-cell lymphoma patients were divided into TRIP13-negative group(0) and TRIP13-positive group(≥1+). According to the intensity of TRIP13immunohistochemical expression, TRIP13-positive patients were further divided into TRIP13 weakly positive group(1+) and TRIP13 strong positive group(≥2~+). The gender, age, B-cell lymphoma type, proliferation index(Ki-67), lactate dehydrogenase, β2microglobulin, albumin levels, proportion of T helper cells, proportion of activated CD4~+cells, activated CD8~+cells and CD4~+/CD8~+ratio were compared among different groups. Results The levels of TRIP13 m RNA in the indolent lymphoma group and the aggressive lymphoma group were 0.37(0.17, 1.36) and 0.69(0.28, 1.15), respectively(P >0.05). The TRIP13m RNA expression level, Ki-67 level and the percentage of activated CD8~+cells in the TRIP13-positive group were higher than those in the TRIP13-negative group, while the percentage of T helper cells and the percentage of activated CD4~+cells,CD4~+/CD8~+ratio were lower than those in the TRIP13-negative group(all P<0.05). The TRIP13 m RNA expression level, Ki-67 level, the percentage of activated CD8~+cells in the TRIP13 weakly positive group were all lower than those in the TRIP13 strong positive group, while the percentage of activated CD4~+cells and CD4~+/CD8~+ratio were higher than those in the TRIP13strong positive group(all P<0.05). Conclusion High expression of TRIP13 in B-cell lymphoma tissues has high Ki-67 level, high percentage of activated CD8~+cell and low percentage of activated CD4~+cells, indicating that TRIP13 is associated with tumor cell proliferation and cellular immune environmental anomalies.更多还原