【摘要】 目的 验证光遗传技术能否实现大鼠在体心房颤动（简称房颤）终止。方法 选取12只SD大鼠经右颈静脉系统性注射50μl重组腺相关病毒[rAA V9-CAG-hChR2 （XXM）-eYFP]。7周后，大鼠随机分为房颤组和对照组，每组6只。房颤组大鼠经尾静脉注射Ach-CaCl₂混合物（Ach 66 ug/kg, CaCl₂ 10 mg/kg）,连续给药7天。对照组使用等量PBS液代替Ach-CaCl₂混合溶液。病毒注射8周后，大鼠经麻醉后开胸充分暴露右心房，使用S₁ S₁刺激心房并记录单相动作电位（MAP）和体表心电图（ECG）,分析并统计RR间期、P波幅度、心率及APD₉₀。然后用频率为8 Hz的473 nm蓝光脉冲照射右房，并同步记录光照输出信号和体表心电图，寻找能完全夺获心房节律的最小光功率密度。大鼠通过Burst刺激（6 V,波宽20 ms,持续2～4 s）诱发房颤后，对心房施加4个间隔1 s的光脉冲，观察光照能否有效终止房颤。实验结束后对心房组织进行HE染色、Masson三色染色和免疫荧光染色观察。结果 与对照组比较，房颤组RR间期延长，P波幅度、心率和APD₉₀均减少（P均<0.05）.完全夺获心率的最小光照功率密度为（0.057±0.016） MW/mm²。光照后房颤终止率为（80.7%±5.8%）远高于未光照的（30%±8.6%）;光照后房颤的持续时间为（10.9±1.1）s远低于未光照的（41.6±10.4）s。免疫荧光染色显示转染目标光敏感蛋白ChR2成功。结论 光遗传技术可以有效地终止房颤并减少持续时间。更多还原

【Abstract】 Objective To verify whether cardiac optogenetics can terminate atrial fibrillation（AF） in vivo in SD rat. Methods Twelve SD rats were injected of 50 μl of recombinant adeno-associated virus [rAAV9-CAG-hChR2（XXM）-eYFP] via right jugular vein systemically. After seven weeks, AF group was injected Ach-CaCl₂ mixture（Ach 66 ug/kg, CaCl₂ 10 mg/kg） via tail vein for 7 d, control group was injected an equivalent volume of PBS within the identical performance. Eight weeks post to the AAV injection, the right atria of anesthetized rats, after open-chest, were performed S₁S₁ stimulation（at 150 ms-baseline）, and monophasic action potential（MAP） and body surface electrocardiogram（ECG） were recorded. Then the right atria were irradiated with 473 nm blue light at 8 Hz frequency, the output signal of light and surface electrocardiogram（ECG） were recorded simultaneously to find the minimum light power density that could completely capture the atrial rhythm. Four-light pulses were applied with a delay of 1 second to AF group, after AF was induced by burst stimulation（6 V, Duration 20 ms, lasting 2-4 s）. HE staining, Masson′s trichrome staining and immunostaining observation were performed at the end of all experiments. Results The minimum illumination intensity that could completely capture heart rate was（0.057±0.016） MW/mm².The termination rates with or without illumination were（80.7%±5.8%）and（30%±8.6%）, respectively, the recovery times with or without illumination were（10.9±1.1）s and（41.6±10.4）s. Immunostaining showed the successful transfection of ChR2. Conclusion Cardiac optogenetics can terminate AF in ChR2-expressing rats effectively.[Chinese Journal of Cardiac Pacing and Electrophysiology,2022,36（3）:223-227]更多还原