【摘要】 目的 根据隔绝空气抑制细菌生长的原理，研究植物油用作24 h尿蛋白定量标本保存防腐剂的应用效果。方法 选取2020年11月至2021年9月深圳市宝安区中医院进行尿常规和尿有形成分分析的标本30份作为研究对象，采用尿液干化学分析尿蛋白半定量结果±、1+、2+、3+、4+为筛选标准，每个梯度选取6份。采用分样试验将其分为试验组和比对组，各30份。比对组即时用生化分析仪检测其尿蛋白定量结果并作为比对值，试验组用植物油将标本做液面封闭隔绝空气，放置室温24 h，再次用生化分析仪检测其尿蛋白定量结果作为试验值，比较两组尿蛋白定量结果的差异。结果 试验组和比对组尿蛋白定量比较，差异无统计学意义（P>0.05）。相对偏倚为（-1.37±8.25）%，两组数据比较差异无统计学意义（P>0.05）。参照CLSI指南EP15-A2文件中运用患者标本验证两种方法的准确度的方案，计算出试验组和比对组相对偏倚均值绝对值1.37%，小于临床实验室所用1/2允许总误差标准22%。结论 植物油可以用于24 h尿蛋白定量标本常温保存，植物油防腐剂具有材料易得，经济安全，操作方便的优点，适于推广使用。更多还原

【Abstract】 Objective According to the principle of isolating air and inhibiting bacterial growth, the application effect of vegetable oil as preservative for the preservation of 24 h urine protein quantitative specimens was studied. Methods From November 2020 to September 2021, 30 samples of urine routine and urine visible component analysis in Shenzhen Bao′an Traditional Chinese Medicine Hospital were selected as the research subjects. The semi-quantitative results of urine protein were ±, 1+, 2+, 3+ and 4+ as screening criteria, and 6 samples were selected from each gradient.The samples were divided into experimental group and comparison group with 30 samples in each group. The quantitative results of urine protein in the comparison group were detected by biochemical analyzer and used as the comparison value, In the experimental group, the samples were sealed with vegetable oil as liquid level and isolated from air. The samples were placed at room temperature for 24 h, and the quantitative results of urinary protein were detected by biochemical analyzer again as the test value, the quantitative results of urinary protein were compared between the two groups. Results There was no significant difference in urine protein between experimental group and comparison group(P>0.05). The relative bias was(-1.37±8.25) %, and there was no significant difference between the two groups(P>0.05). According to the scheme in the CLSI guideline EP15-A2 of using patient specimens to verify the accuracy of the two methods, the absolute value of the relative bias of the test group and the comparison group was calculated to be1.37%, which was less than the 1/2 allowable total error standard used by clinical laboratories, 22%. Conclusion Vegetable oil can be used for 24 h urine protein quantitative sample storage at room temperature, vegetable oil preservatives have the advantages of easy material, economic safety, convenient operation, suitable for popularization.更多还原