【摘要】 目的 探究重组人酸性成纤维生长因子（RhaFGF）对糖尿病大鼠难愈性皮肤溃疡愈合的影响，并进一步研究RhaFGF对糖尿病大鼠难愈性皮肤溃疡的修复作用及机制。方法 建立糖尿病难愈性皮肤溃疡大鼠模型，将28只大鼠随机分为对照组和RhaFGF组，对照组采用生理盐水局部外敷，RhaFGF组采用RhaFGF局部外敷。于第7、14天记录创面面积，并取大鼠皮肤溃疡处新生肉芽组织，检测超氧化物歧化酶（SOD）、丙二醛（MDA）、血小板内皮细胞黏附分子（CD31）、细胞核增殖抗原（Ki-67）及病理切片，以对RhaFGF治疗糖尿病难愈性溃疡的治疗效果进行综合评价。结果 治疗第7、14天，RhaFGF组的溃疡创面面积小于对照组，差异有统计学意义（P<0.05）;RhaFGF组的MDA含量低于对照组，差异有统计学意义（P<0.05）;RhaFGF组的SOD活性高于对照组，差异有统计学意义（P<0.05）;RhaFGF组的CD31阳性表达率高于对照组，差异有统计学意义（P<0.01）;RhaFGF组的Ki-67阳性表达率高于对照组，差异有统计学意义（P<0.01）。组织病理学检查显示RhaFGF组新生的毛细血管、成纤维细胞数量和胶原蛋白表达量较治疗前增加。结论 RhaFGF通过促进糖尿病难愈性溃疡的氧化应激，增加溃疡组织的毛细血管和成纤维细胞生成，提高上皮再生水平，从而表现出对大鼠糖尿病溃疡组织的修复作用。更多还原

【Abstract】 Objective To investigate the effect of recombinant human acidic fibroblast growth factor(RhaFGF) on healing of diabetic rats with refractory skin ulcers, and to further study the effect and mechanism of RhaFGF on the healing of diabetic rats with refractory skin ulcers. Methods The diabetic rat model of skin ulcer was established. A total of 28 rats were randomly divided into control group and RhaFGF group. The control group was treated with topical saline, and RhaFGF group was treated with topical application of RhaFGF. The wound area was recorded on the seventh day and the fourteenth day, and the new granulation tissue in the skin ulcer area of rats was collected. Superoxide dismutase(SOD), malondialdehyde(MDA), platelet endothelial cell adhesion molecule(CD31), functional antigen Ki-67(Ki-67)and pathological sections were examined to evaluate the therapeutic effect of RhaFGF in treating diabetic refractory ulcers. Results On day 7 and 14 of treatment, the wound area of the RhaFGF group was smaller than that of the control group, and the difference was statistically significant(P<0.05). MDA content in the RhaFGF group was lower than that in the control group, and the difference was statistically significant(P<0.05). SOD activity in the RhaFGF group was higher than that in the control group, and the difference was statistically significant(P<0.05). The positive expression rate of CD31 in the RhaFGF group was higher than that in the control group, and the difference was statistically significant(P<0.01). The positive expression rate of Ki-67 in the RhaFGF group was higher than that in the control group, and the difference was statistically significant(P<0.01). Histopathological examination showed that the number of new capillaries, fibroblasts and collagen expression increased in RhaFGF group compared with before treatment. Conclusion RhaFGF promotes oxidative stress of diabetic refractory ulcers, increases the production of capillaries and fibroblasts in ulcerated tissues, and significantly increases the level of epithelial regeneration, thereby demonstrating the repair effect on diabetic ulcers.更多还原