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竭香定痛胶囊治疗冠心病网络药理学作用机制研究

Mechanism study of Jiexiang Dingtong Capsules in the treatment of coronary heart disease based on network pharmacology

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【作者】 曹宝国刘惠娟王新舜王恒马琳樊红波

【Author】 CAO Baoguo;LIU Huijuan;WANG Xinshun;WANG Heng;MA Lin;FAN Hongbo;Department of Internal Medicine of Traditional Chinese Medicine, Tianshui Hospital of Traditional Chinese Medicine,Gansu Province;Department of Pharmacy, Tianshui Hospital of Traditional Chinese Medicine,Gansu Province;Department of Cardiology, Tianshui Hospital of Traditional Chinese Medicine,Gansu Province;

【机构】 甘肃省天水市中医医院中医内科甘肃省天水市中医医院临床药学研究室甘肃省天水市中医医院心内科

【摘要】 目的利用网络药理学方法分析竭香定痛胶囊治疗冠心病的作用机制。方法在中药系统药理学数据库(TCMSP)筛选竭香定痛胶囊的有效成分及作用靶点,在OMIM数据库及GeneCards数据库中检索冠心病基因靶点,应用R语言映射竭香定痛胶囊-冠心病共有基因,应用Cytoscape软件和STRING数据库建立中药调控和蛋白质相互作用(PPI)网络,应用bioconductor中"DOSE""clusterProfiler"及"pathview"程序包在R语言中对共有基因进行基因本体(GO)功能富集及基因组百科全书(KEGG)通路富集。结果筛选出竭香定痛胶囊的36个有效成分及804个基因,其中100个基因是与冠心病共有基因;PPI核心网络包括100个蛋白及390条边,关键基因涉及丝裂原激活蛋白激酶8(MAPK8)、表皮生长因子受体(EGFR)、白细胞介素-6(IL-6)、雌激素受体基因1(ESR1)及血管内皮生长因子A(VEGFA);GO富集分析结果涉及核受体的活动、类固醇激素受体活性、氧化还原酶活性及雌激素受体结合等;KEGG通路富集有PI3K-Akt信号通路、液体剪切应力和动脉粥样硬化通路、细胞凋亡通路及肿瘤坏死因子信号通路等。结论竭香定痛胶囊治疗冠心病时,存在多分子、多基因靶点及多通路协作机制,可能通过调控MAPK8基因蛋白表达,影响类固醇激素受体活性、氧化还原酶活性及雌激素受体,并调控PI3K-Akt信号通路、液体剪切应力和动脉粥样硬化通路、细胞凋亡通路及肿瘤坏死因子信号通路等多种方式治疗冠心病。

【Abstract】 Objective To analyze the mechanism of Jiexiang Dingtong Capsules in the treatment of coronary heart disease by network pharmacology. Methods To screen the effective components and targets of Jiexiang Dingtong Capsules in traditional Chinese medicine systems pharmacology databaseand analysis platform(TCMSP), the coronary heart disease gene targets were retrieved in OMIM database and GeneCards database, R language was used to map the common genes of Jiexiang Dingtong Capsules-coronary heart disease, Cytoscape software and STRING database were used to establish the traditional Chinese medicine regulatory and protein-protein interaction(PPI) network. To perform gene ontology(GO) function and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment of common genes in R language by using bioconductor "DOSE" "clusterProfiler" and "pathview" packages. Results A total of 36 active components and 804 genes of Jiexiang Dingtong Capsules were screened, 100 genes were common genes with coronary heart disease. PPI core network consists of 100 proteins and 390 edges. Key proteins involved mitogen-activated protein kinase 8(MAPK8), epidermal growth factor receptor(EGFR), intedeukin-6(IL-6), estrogen receptor gene 1(ESR1)and vascular endothelial growth factor(VEGFA). The results of GO enrichment analysis involved nuclear receptor activity, steroid hormone receptor activity, redox enzyme activity and estrogen receptor activity, etc. KEGG pathway were enriched with PI3 K-Akt signaling pathway, fluid shear stress and atherosclerosis pathway, cell apoptosis pathway, tumor necrosis factor signaling pathway, etc. Conclusion There are multi-molecular and multi-gene targets and multipathway cooperative mechanisms in Jiexiang Dingtong Capsules in the treatment of coronary heart disease. It may affect steroid hormone receptor activity, oxidoreductase activity and estrogen receptor by regulating MAPK8 gene protein expression, and regulate PI3 K-Akt signaling pathway, liquid shear stress and atherosclerosis pathway, cell apoptosis pathway, tumor necrosis factor signaling pathway to treat coronary heart disease.

  • 【文献出处】 中国当代医药 ,China Modern Medicine , 编辑部邮箱 ,2022年01期
  • 【分类号】R285
  • 【被引频次】1
  • 【下载频次】120
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