【摘要】 以东方亮1号小米为原料,研究小米多酚的体外抗氧化活性和降血糖活性。对纯化前后的小米多酚进行DPPH自由基清除率、羟基自由基清除率、还原力、对铁离子的络合作用和总抗氧化能力(T-AOC)的评价以及抑制α-葡萄糖苷酶、α-淀粉酶活性的实验,同时测定粗提物与纯化物对葡萄糖的吸收能力。结果表明,抗氧化能力均为阳性对照>小米多酚纯化物>小米多酚粗提物。纯化后的小米多酚对α-淀粉酶有较好的抑制效果,纯化物和粗提物均对α-葡萄糖苷酶的抑制率较低,抑制效果较差。小米多酚对葡萄糖有一定的吸收能力,且纯化物强于粗提物。更多还原

【Abstract】 Using Dongfangliang No.1 millet as the raw material, the antioxidant activity and hypoglycemic activity in vitro of millet polyphenols are studied. The DPPH free radical scavenging rate, hydroxyl free radical scavenging rate, reducing power, complexation to iron ions and total antioxidant capacity(T-AOC) of millet polyphenols before and after purification are evaluated, and the experiments for inhibiting the activities of α-glucosidase and α-amylase are carried out. At the same time, the absorption capacity of crude extract and purified extract to glucose is determined. The results show that the antioxidant capacity is positive control>millet polyphenol purified extract>millet polyphenol crude extract. The purified millet polyphenols have a good inhibitory effect on α-amylase, while the purified and crude extracts have lower inhibitory rates on α-glucosidase, and the inhibitory effect is poor. The millet polyphenols have certain absorption ability to glucose, and the purified extract is stronger than the crude extract.更多还原