【摘要】 目的 探讨1,25-二羟基维生素D₃[1,25（OH）₂D₃]对DM小鼠周围神经功能的影响。方法C57BL/6J小鼠腹腔注射STZ构建DM小鼠模型，随机分为DM组（DM）、DM第1周1,25（OH）₂D₃治疗组（1w-DM）、DM第2周1,25（OH）₂D₃治疗组（2w-DM）、DM周围神经病变1,25（OH）₂D₃治疗组（DPN）以及DM第1周溶剂对照组（1w-DMC）、DM第2周溶剂对照组（2w-DMC）、DPN溶剂对照组（DPNC），每组各15只，并设置正常对照组（NC）10只。建模后第1、2、4周分别对1w-DM、2w-DM、DPN组予1,25（OH）₂D₃腹腔注射，连续5 d；监测各组血糖、体重、触觉、热痛觉及协调能力，8周后收集血清检测25（OH）D₃水平。结果 与DM组比较，1w-DM、2w-DM组第8周体重升高（P<0.05），血糖降低（P<0.05），协调运动时间、血清25（OH）D₃水平升高（P<0.05或P<0.01），触觉反应时间、热痛觉反应时间更短（P<0.05）。结论 在DM神经功能受损前，1,25（OH）₂D₃治疗可有改善DM小鼠周围神经功能。更多还原

【Abstract】 Objective To evaluated the effects of 1,25-dihydroxyvitamin D₃[1,25（OH）₂D₃]on peripheral nerve function in DM mice.Methods C57BL/6J mice were intraperitoneally injected with STZ to establish DM mouse model. They were randomly divided into DM group（DM）,1st week 1,25（OH）₂D₃treatment group（1w-DM）,2nd week 1,25（OH）₂D₃treatment group（2w-DM）,diabetic peripheral neuropathy1,25（OH）₂D₃treatment group（DPN）,1st week DM solvent control group（1w-DMC）,2nd week DM solvent control group（2w-DMC）,DPN solvent control group（DPNC）with 15 in each groups,and 10 in normal control group（NC）. 1w-DM group,2w-DM group and DPN group were intraperitoneally injected with 1,25（OH）₂D₃for 5 consecutive days at week 1,2 and 4 after model construction. Blood glucose,body weight,touch,heat pain sensation and coordination ability of mice were monitored in each group for 8 weeks.After 8 weeks,serum was collected for 25（OH）D₃testing.Results After 8 weeks,compared with DM group,the body weight increased,blood glucose decreased（P<0. 05）,coordinated motion time and serum25（OH）D₃level increased（P<0. 05 or P<0. 01）,tactile response time and heat pain response time were shorter in 1w-DM and 2w-DM groups（P<0. 05）.Conclusion 1,25（OH）₂D₃treatment can improve peripheral nerve function in DM mice before neurological impairment.更多还原