【摘要】 南极磷虾油富含虾青素和不饱和脂肪酸,是南极大磷虾提取物中具有较高开发价值的产品之一,然而,其在水中的难溶性限制了其在食品中的应用。壳聚糖作为一种高分子多糖,常被用作一些药物的载体,可以达到控制药物释放,增强疗效,减少不良反应的作用。本文通过响应面优化法研究磷脂胆固醇比、磷脂磷虾油比和壳聚糖共轭质量分数3个因素对壳聚糖-磷虾油纳米脂质体共轭物的影响,并探究优化产物对丙烯酰胺诱导的斑马鱼胚胎氧化应激模型在生长发育方面的影响。结果表明:在卵磷脂∶胆固醇为5∶1,卵磷脂∶磷虾油为19.6∶1,壳聚糖质量分数为0.146%的条件下,粒径适中为145 nm,且包封率最高为98.1%。此外,其对氧化应激损伤造成的生长发育抑制,具有明显的改善作用,且在一定范围内呈剂量依赖性。更多还原

【Abstract】 Antarctic krill oil is rich in astaxanthin and unsaturated fatty acids. It is one of the products with high development value in Antarctic krill extract. However, its insoluble water solubility limits its application in food. As a polymer polysaccharide, chitosan is often used as a carrier for some drugs, which can control its release, enhance curative effect, and reduce adverse reactions. In this paper, the response surface optimization method was used to optimize the effects of the three factors of phospholipid-cholesterol ratio, phospholipid-krill oil ratio and chitosan conjugate concentration, and further explore the effects of the optimized product on propylene. Effects of amide-induced oxidative stress on the growth and development of zebrafish embryos. The results showed that under the condition that lecithin ∶ cholesterol is 5 ∶ 1, lecithin ∶ krill oil is 19.6 ∶ 1, and the chitosan mass fraction is 0.146%, the particle size is moderately 145 nm, and the maximum encapsulation efficiency is 98.1%. In addition, it has a significant improvement effect on the inhibition of growth and development caused by oxidative stress damage, and it is dose-dependent within a certain range.更多还原