【摘要】 目的:研究丙磺舒（PROB）对血小板衍生生长因子-BB（PDGF-BB）诱发大鼠肺动脉平滑肌细胞的迁移与增殖的影响。方法:体外原代培养SD大鼠肺动脉平滑肌细胞（PASMCs）,随机分为正常对照组（CON组）、造模组（10 ng/ml PDGF-BB 24 h）、丙磺舒处理组（10 ng/ml PDGF-BB和200μmol/L PROB孵育24 h, PROB为Pannexin-1的特异性阻断剂）。通过CCK-8法选取丙磺舒和PDGF-BB干预浓度，并检测各组细胞的增殖能力；通过细胞划痕实验和Transwell^TM实验检测各组PASMCs的迁移能力；通过细胞免疫荧光技术检测各组PASMCs中骨桥蛋白（OPN）和增殖细胞核抗原（PCNA）的分布和表达；通过Western blot法检测各组PASMCs中OPN和PCNA的蛋白水平差异。结果:与CON组相比，PDGF-BB组的PASMCs迁移和增殖能力均有所增强（P<0.05）,经PROB干预后，细胞迁移和增殖能力降低（P<0.05）;与CON组相比，PDGF-BB组OPN和PCNA的表达和蛋白水平均显著升高（P<0.05）,经PROB干预后，其表达和蛋白水平显著降低（P<0.05）。结论:丙磺舒通过阻断Pannexin-1可以抑制PDGF-BB诱发PASMCs的增殖和迁移。更多还原

【Abstract】 Objective: To investigate whether probenecid（PROB） could improve the proliferation and migration ability of rats’ pulmonary artery smooth muscle cells induced by platelet-derived growth factor-BB（PDGF-BB）. Methods: Primary pulmonary artery smooth muscle cells（PASMCs） of SD rats were cultured in vitro, and were randomly divided into control group（CON group）, PDGF-BB group（10 ng/ml PDGF-BB treatment for 24 h） and PDGF-BB+PROB group（10 ng/ml PDGF-BB and 200 μmol/L PROB treatment for 24 h, PROB is a specific blocker of pannexin-1）. CCK-8 method was used to select the suitable intervention concentrations of PROB and PDGF-BB, and to detect the proliferation of PASMCs in each group. The migration ability of PASMCs was detected by Transwell^TM assay and cell scratch test. Immunofluorescence cytochemistry and Western blot were used to detect the protein expressions and distribution of osteopontin（OPN） and proliferating cell nuclear antigen（PCNA） in PASMCs. Results: Compared with CON group, the migration and proliferation ability of PASMCs in PDGF-BB group were enhanced（P<0.05）. After treated with PROB, the migration and proliferation ability of PASMCs in PDGF-BB+PROB group were decreased significantly（P<0.05）. Compared with CON group, the expression and protein levels of OPN and PCNA in PDGF-BB group were increased significantly（P<0.05）, while the expression and protein levels of OPN and PCNA in PDGF-BB+PROB were decreased significantly（P<0.05）. Conclusion: Probenecid inhibits the migration and proliferation of PDGF-BB-induced PASMCs by blocking Pannexin-1.更多还原