【摘要】 目的：探讨早期跑步运动是否通过激活内皮型一氧化氮合酶（activating endothelial nitric oxide synthase,e NOS）在大鼠缺血/再灌注（ischemia/reperfusion,I/R）损伤中发挥保护作用。方法：50只雄性SD大鼠随机分为5组：Sham组（n=10）、I/R组（n=10）、运动+I/R组（n=10）、eNOS抑制剂（L-NIO）+I/R组（n=10）和运动+L-NIO+I/R组（n=10）。I/R大鼠行大脑中动脉阻塞（middle cerebral artery occlusion,MCAO）60mins，再灌2天后，运动+I/R组和运动+L-NIO+I/R组大鼠在跑步机上连续跑5天，每次30mins。再灌7天后，评估各组大鼠神经功能、脑梗死体积，以及梗死周围区和脑血管P-eNOS和P-AMPK蛋白表达。结果：运动+I/R组神经行为学评分及脑梗死体积明显低于I/R组、L-NIO+I/R组和运动+L-NIO+I/R组（所有P<0.05）。与I/R组、L-NIO+I/R组和运动+L-NIO+I/R组相比，运动+I/R组梗死周围区和脑血管P-eNOS和P-AMPK蛋白水平较高（所有P<0.05）。结论：早期跑步运动可能通过激活eNOS减轻I/R大鼠神经血管损伤，改善功能预后。更多还原

【Abstract】 Objective:To investigate whether early running could protect rats from ischemia/reperfusion(I/R) injury by activating endothelial nitric oxide synthase(eNOS).Method:Fifty male SD rats were randomly divided into five groups:Sham group(n=10),I/R group(n=10),exercise + I/R group(n=10),eNOS inhibitor(L-NIO) + I/R group(n=10) and exercise + L-NIO + I/R group(n=10). I/R rats were subjected to middle cerebral artery occlusion(MCAO) for 60 minutes,and then perfused for 2 days. Rats in exercise + I/R and exercise + L-NIO + I/R group ran continuously on treadmill for 5 days,30 minutes each time. After 7 days of reperfusion,the neurological score,cerebral infarction volume,P-eNOS and p-AMPK protein expression in ischemic penumbra and cerebral vessels were evaluated.Result:The neurobehavioral score and cerebral infarction volume in exercise + I/R group were significantly lower than those in I/R group,L-NIO + I/R group and exercise + L-NIO + I/R group(all P<0.05). Compared with I/R group,L-NIO + I/R group and exercise + L-NIO + I/R group,P-eNOS and p-AMPK protein levels in ischemic penumbra and cerebral vessels were higher in exercise + I/R group(all P<0.05).Conclusion:Early running may reduce neurovascular injury and improve functional prognosis of I/R rats by activating eNOS.更多还原