【摘要】 目的 制备靶向诊疗一体化脂质体纳米粒(FA-PFH-ICG),用于视网膜母细胞瘤Y79细胞的精准诊疗。方法 采用超声双乳化法制备纳米粒，检测其表征并评估体外靶向Y79细胞的能力。研究纳米粒体外增强超声/光声成像及协同杀伤Y79细胞的能力。结果 纳米粒呈大小均一的球形，能大量靶向Y79细胞。在808 nm激光下，纳米粒能增强体外超声及光声成像。光热联合光动力治疗下，纳米粒使大量Y79细胞死亡。结论 新型FA-PFH-ICG纳米粒能够有效靶向视网膜母细胞瘤Y79细胞，实现体外增强超声/光声成像下的协同治疗。更多还原

【Abstract】 Objective To prepare the targeted diagnosis and treatment integrated liposome nanoparticles(FA-PFH-ICG) for the precise diagnosis and treatment of retinoblastoma Y79 cells. Methods Nanoparticles were prepared by ultrasonic double emulsification method to detect the characterization and evaluate the ability to target Y79 cells in vitro. To study the synergistic killing ability of Y79 cells by enhancing ultrasound/photoacoustic imaging with nanoparticles in vitro. Results Nanoparticles were uniformly spherical in size and can target Y79 cells in large numbers. Under 808 nm laser, nanoparticles can enhance ultrasound and photoacoustic imaging in vitro. In combination with photothermal and photodynamic therapy, a large number of Y79 cells were killed by nanoparticles. Conclusions The novel FA-PFH-ICG nanoparticles can effectively target retinoblastoma Y79 cells to achieve synergistic therapy under enhanced ultrasound/photoacoustic imaging in vitro.更多还原