【摘要】 目的 制备一种三模态成像的脂质体纳米粒，评价其体外生物安全性和体内外光声/超声/磁共振成像效果。方法 制备包裹ICG和PFP、载Fe₃O₄的脂质体纳米粒（PFP-ICG-Fe₃O₄-NPs）。检测其相关表征和体外生物安全性。观察PFP-ICG-Fe₃O₄-NPs体内外超声/光声/MRI成像效果。结果 成功制备PFP-ICG-Fe₃O₄纳米粒，电镜下（TEM）表现为形态规则的球形。平均粒径：（335.76±6.55）nm,平均电位：（-30.5±3.84）mV。激光激发下，光声与超声均在体内外有增强信号的效果，磁共振成像体内外均在T2W1呈低信号。结论 成功制备PFP-ICG-Fe₃O₄纳米粒。具有良好的体内外光声/超声/磁共振成像效果。更多还原

【Abstract】 Objective To prepare a tri-model imaging liposome nanoparticles and explore its biosafety and, the effect of photoacoustic, ultrasonic and magnetic resonance imaging in vitro and vivo. Methods Phase variation liposome nanoparticles coated with ICG, PFP and Fe₃O₄（PFP-ICG-Fe₃O₄-NPS） were prepared. The characterization and the biosafety of PFP-ICG-Fe₃O₄ nanoparticles were assessed in vitro. The effects of photoacoustic/ultrasonic/magnetic resonance imaging from PFP-ICG-Fe₃O₄ in vitro and vivo were evaluated with different methods. Results PFP-ICG-Fe₃O₄ were successfully prepared. Spherical particle with regular shape and uniform size was observed by transmission electron microscope（TEM）. Average size and potencial of the target product was measured to be（335.76±6.55）nm and（-30.5±3.84） mV. In vivo and vitro, both photoacoustic and ultrasonic signals were obvious after laser irradiation, and MRI signal of nanoparticles was at low level in T2 W1. Conclusions The PFP-ICG-Fe₃O₄ nanoparticles were prepared. Such particles possess excellent photoacoustic/ultrasonic/magnetic resonance imaging effect in vivo and in vitro.更多还原