【摘要】 目的 应用基于体素的核磁共振（MRI）形态学测量技术及基因检测技术，分析遗忘型轻度认知障碍（aMCI）相对于正常老年人脑灰质体积改变的特点、载脂蛋白E（ApoE）基因多态性并探讨上述改变与aMCI发病机制可能的相关性，为aMCI的早期诊断、早期干预提供依据。方法 临床招募年龄、性别、族别、文化程度、生活背景等相互匹配并确定无血缘关系的14例aMCI患者、15例正常老年人，用3.0T磁共振进行三维T₁WI扫描，利用基于SPM12的DARTEL工具箱对扫描获得的结构图像进行预处理，再对aMCI组和对照组的全脑灰质体积进行基于体素的统计学比较。通过检测静脉血中ApoE基因，鉴定每个样本ApoE基因的等位基因及基因型。将上述所得数据进行相关统计学分析。结果 aMCI组中ε3/ε4基因型与正常对照组相比差异有统计学意义（P<0.05）。aMCI组与对照组比较，脑灰质萎缩主要位于左侧顶上小叶、左侧岛叶、岛叶前部、中央后回、腹内侧前额叶，右侧角回。结论 证实了aMCI患者患病与ApoE基因多态性存在联系，ApoEε4可能为aMCI的危险因素，aMCI患者灰质萎缩主要位于顶上小叶、岛叶。更多还原

【Abstract】 Objective to analyze the characteristics of brain gray matter volume changes and ApoE gene polymorphism in aMCI patients with compared with normal elderly people by voxel-based MRI morphometry and gene detection technology. Exploring the possible correlation in those changes and the pathogenesis of aMCI. Methods Recruiting 14 aMCI patients and 15 normal elderly people who were matched with each other by age, gender, ethnicity, education, living background and determined no blood relationship. To give every case three-dimensional T₁WI scan with 3.0 T magnetic resonance imaging. We use the SPM12-based DARTEL toolbox to preprocess the structural images obtained from the scan, and then compare voxel-based statistical results of the whole brain gray matter volume of the aMCI group and the control. Detecting the ApoE gene by drawing blood to identify the allele and genotype of the ApoE gene in each sample. The data obtained was analyzed by SPSS20.0 software to calculate the allele frequency and genotype frequency of the three groups of ApoE genes. Results The ε3/ε4 genotype in the group aMCI was significant difference between the aMCI and the control（P<0.05）. Compared with the control, the gray matter atrophy of the aMCI was mainly located in the left insula, superior frontal gyrus, middle frontal gyrus, anterior insula, posteriorcentral gyrus, ventromedial prefrontal lobe, bilateral superior parietal lobule, right angular gyrus. Conclusion ApoE gene polymorphism was associated with the morbidity of patients with aMCI,and ApoEε4 may be a risk factor for aMCI. The thalamus, posterior cingulate cortex were also involved. Gray matter atrophy in patients with aMCI was mainly located in the parietal lobule and the insular.更多还原