【摘要】 目的 建立苁蓉连翘合剂的质量标准。方法 采用薄层色谱（TLC）法对样品中肉苁蓉、连翘、佛手进行定性鉴别；采用高效液相色谱（HPLC）法测定样品中松果菊苷的含量，色谱柱为Agilent Zorbax Eclipse XDB-C18柱（250 mm×4.6 mm,5μm），流动相为甲醇-0.1%甲酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为330 nm，柱温为30℃。结果 肉苁蓉、连翘、佛手的TLC斑点清晰，分离度好，阴性对照无干扰。松果菊苷进样量在1.0～20.0μg范围内与峰面积线性关系良好（r=0.999 9,n=6）；精密度、稳定性、重复性试验的RSD均小于1.1%，平均加样回收率为98.76%,RSD为2.05%(n=9)。结论 所建立的方法简便易行，专属性强，准确可靠，可用于苁蓉连翘合剂的质量控制。更多还原

【Abstract】 Objective qualitative identification of Cistanches Herba,Forsythiae Fructus and Citri Sarcodactylis Fructus in samples were identified by thin-layer chromatography(TLC). The content of echinacoside was determined by high-performance liquid chromatography(HPLC)method,the chromatographic column was Agilent Zorbax Eclipse XDB-C18column(250 mm × 4. 6 mm,5 μm),the mobile phase was methanol-0. 1% formic acid solution(gradient elution),the flow rate was 1. 0 mL/min,the detection wavelength was 330 nm,the column temperature was 30 ℃.ResultsThe TLC spots of Cistanches Herba,Forsythiae Fructus and Citri Sarcodactylis Fructus were clear and well separated,and the negative sample had no interference. The injection volume of echinacoside showed a good relationship with the peak area in the range of 1. 0-20. 0 μg(r = 0. 999 9,n = 6). The RSDs of precision,stability and repeatability tests were less than 1. 1%,the average recovery was 98. 76% with RSD of 2. 05%(n = 9).Conclusion with strong specificity is simple,accurate,reliable,and it can provide a reference for the quality control of Cistanches Herba and Forsythiae Fructus mixture.更多还原