【摘要】 目的 建立严重急性呼吸综合征冠状病毒2(SARS-CoV-2)Spike(S)蛋白的快速检测方法。方法 基于抗原-抗体免疫识别反应、纳米技术、光敏水凝胶技术，制备同时偶联抗体和光敏剂的纳米粒子溶液，SARS-CoV-2表面的S蛋白可与纳米粒子上的抗体形成牢固的非共价结合，去除其余干扰性结合后，在波长为405 nm的蓝光和光引发剂Irgacure 2959的作用下，S蛋白可使甲基丙烯酰化明胶（GelMA）水凝胶溶液由液态转变为固态。结果 制备的检测溶液中，S蛋白抗体质量为50～100 mg，疏基-Irgacure 2959质量为5～10 mg。同时，当GelMA水凝胶溶液的浓度为5%～30%(m/V)时，检测效果较好。结论 该方法生产成本低，检测效率高，易于贮存和运输，对设备和操作人员的依赖性低，可用于快速检测SARS-CoV-2表面的S蛋白。更多还原

【Abstract】 Objective To establish a rapid detection method for the Spike(S) protein of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).MethodsBased on antigen-antibody immunorecognition response,nanotechnology and photosensitive hydrogel technology,nanoparticle solution with simultaneous coupling of antibody and photosensitizer was prepared.The S protein on the surface of SARS-CoV-2 could form a strong non-covalent binding with the antibody on the nanoparticle,and after the remaining interfering binding was removed,gelatin methacryloyl(GelMA) hydrogel solution would be changed from liquid to solid under the action of blue light of 405 nm and photoinitiator Irgacure 2959 due to the S protein.ResultsIn the prepared detection solution,the mass of S protein antibody was 50-100 mg and the mass of HS-irgacure 2959 was 5-10 mg. At the same time,when the concentration of GelMA hydrogel solution was 5%-30%(m/V),the detection effect was good.ConclusionThe method has low production cost,high detection efficiency,easy storage and transportation,low dependence on equipment and operator,which can be used for rapid detection of the S protein on the surface of SARS-CoV-2.更多还原