【摘要】 目的 建立高效液相色谱法同时测定不同产地当归中4种苯酞类成分的含量并对其进行聚类分析，评价其质量。方法 采用Agilent Eclipse Plus C18（4.6 mm×150 mm,3.5μm）色谱柱；流动相为乙腈-水，梯度洗脱；柱温35℃；检测波长：洋川芎内酯I、洋川芎内酯A为284 nm，藁本内酯为326 nm，丁烯基苯酞为260 nm；流速1 ml/min。运用SPSS 26.0分析软件进行聚类分析。结果 4种成分均能达到基线分离，各成分线性关系良好（r=0.9999），线性范围分别为洋川芎内酯I 5.85×10^-3～0.234μg、洋川芎内酯A 1.12×10^-2～0.446μg、藁本内酯0.416～16.64μg；丁烯基苯酞5.20×10^-3～0.208μg；平均加样回收率为98.33%～102.22%,RSD为1.36%～3.61%。不同产地的当归按不同部位的含量可分别分为两大类，对于当归头而言，云南曲靖1、云南白药基地1和云南怒江2三个产地的为一类，其余产地的为一类；对于当归身而言，则云南曲靖1、云南怒江2、湖北神农架和云南白药基地1四个产地的为一类，其余产地的为一类。结论 该法简便、准确、专属性强，可用于当归中4种苯酞类成分的含量测定，为当归药材的质量评价和综合开发利用提供科学依据。更多还原

【Abstract】 Objective To establish an high performance liquid chromatography method for content determination of four kinds of phenylphthalides in Radix Angelicae Sinensis, and to evaluate its quality by cluster analysis. Methods The Agilent Eclipse Plus C18column（4.6 mm×150 mm, 3.5 μm） was used as the column. The mobile phase was composed of acetonitrile-water with gradient elution at a flow rate of 1 ml/min. The column temperature was 35℃. The detection wavelength were set at 284 nm for senkyunolide I and senkyunolide A, 326 nm for ligustilide, and 260 nm for 3-butylidenephthalide. SPSS 26.0 statistical software was performed on cluster analysis. Results All the four components could achieve baseline separation with good liner relationships（r = 0.9999）, and the calibration curves of senkyunolide I,senkyunolide A, ligustilide and 3-butylidenephthalide were in good linearity over the range of 5.85 ×10^-3to 0.234 μg,1.12×10^-2to 0.446 μg, 0.416 to 16.64 μg, 5.20 ×10^-3to 0.208 μg. The average recoveries were between 98.33% and 102.22%, with the RSD between 1.36% and 3.61%. The samples of Radix Angelica Sinensis from different habitats could be divided into two species according to the content in different parts. For Radix Angelicae Sinensis head, the ones from Yunnan Qujing 1, Yunnan Baiyao Base 1 and Yunnan Nujiang were grouped together, and the others were grouped together. For Radix Angelicae Sinensis body, the ones from Yunnan Qujing 1, Yunnan Nujiang 2, Hubei Shennongjia, and Yunnan Baiyao Base 1 were grouped together, and the others were grouped together. Conclusion The method is simple, accurate and specific, for the determination of four phenylphthalides in Radix Angelicae Sinensis,which can provide reference for the quality evaluation and comprehensive development and utilization of Angelica Sinensis.更多还原