【摘要】 目的 探究白细胞（WBC）、红细胞（RBC）、尿肌酐（Ucr）指标变化与尿液标本不同保存条件的关系。方法 选取2021年1月至2021年8月期间郑州市第一人民医院180例健康体检者作为研究对象，均采集中段尿液标本，分析除温度、时间之外影响尿液标本检验结果的因素；将合格标本等分2份，于不同温度（4℃、25℃）条件下，检测取样即刻、静置1 h、2 h、3 h、4 h、5 h WBC、RBC、Ucr指标变化情况，分析其与保存条件的关系。结果 本组共180份健康体检者尿液标本，排除温度、时间影响，不合格率为18.33%(33/180)，影响因素为采集尿液样本前受检者准备不当、采集尿液过程中受到污染或感染、检验所用仪器保养或操作不当、标本标记不清；合格尿液标本中WBC不受温度、时间等保存条件的影响；4℃、25℃条件下合格尿液标本静置时间≥3 h时，Ucr较静置时间<3 h显著升高，差异有统计学意义（P<0.05），且25℃升高幅度明显大于4℃，差异有统计学意义（t=29.049、29.681、29.598,P<0.05）;4℃、25℃条件下合格尿液标本静置时间≥3 h时，RBC较静置时间<3 h显著下降，差异有统计学意义（P<0.05），且25℃降低幅度明显大于4℃，差异有统计学意义（t=20.005、11.627、10.136,P<0.05）。结论 尿液标本检验结果受患者准备不当、仪器、操作等多种因素影响，且合格尿液标本随着存放时间的延长、温度变化，RBC、Ucr水平发生显著变化，因此为确保检测结果准确度，取样后应尽量在3 h内完成检测，并尽量在4℃环境下保存。更多还原

【Abstract】 Objective To investigate the relationship between changes of white blood cell(WBC),red blood cell(RBC)and urine creatinine(Ucr)indexes and different storage conditions of urine samples. Methods 180 healthy subjects from our hospital from January 2021 to August 2021 were selected as the research subjects. The middle urine samples were collected to analyze the factors affecting the urine sample test results except temperature and time. Two parts were aliquoted,and the changes of WBC,RBC and Ucr were measured immediately at different temperatures(4℃,25℃)for 1 h,2 h,3 h,4 h,5 h,and analyzed.Save the relationship of the conditions. Results A total of 180 urine samples from healthy subjects were excluded from temperature and time. The unqualified rate was 18.33%(33/180). The influencing factors were improper preparation and urine collection before the urine samples were collected. In case of pollution or infection,improper maintenance or improper operation of the instrument used for inspection,the specimen is unclear;WBC in qualified urine specimens is not affected by storage conditions such as temperature and time;Under the conditions of 4℃ and 25℃,when the qualified urine samples were placed for more than 3 h,the Ucr was significantly increased compared with the rest time of <3 h,and the difference was statistically significant(P<0.05),and the increase range was obvious at 25℃. Above 4℃,the difference was statistically significant(t=29.049,29.681,29.598,P<0.05);when the resting time of qualified urine samples at 4℃ and 25℃ was ≥3h,RBC was less than resting time <3 h decreased significantly,and the difference was statistically significant(P<0.05),and the decrease at 25℃ was significantly greater than that at 4℃,and the difference was statistically significant(t=20.005,11.627,10.136,P<0.05)Conclusion The results of urine specimen test are affected by various factors such as improper preparation,instrument and operation. The qualified urine specimens change significantly with the storage time and temperature,and the RBC and Ucr levels change significantly. Therefore,the test results are accurate. Degree,after sampling,should be completed within 3 h,and try to save at 4℃.更多还原