【摘要】 病原宏基因组高通量测序（m NGs）不依赖于传统的微生物培养，直接对临床样本中的核酸进行高通量测序，具有无偏倚、广覆盖、快速等优点。检测临床样本中的较多病原微生物（包括病毒、细菌、真菌、寄生虫），在重症感染领域，特别是疑难、罕见病导致的重症感染中发挥了关键作用。然而，感染性的临床样本多种多样，样本前处理及核酸提取方法并没有标准规范。各专家共识中也都指出样本前处理和核酸提取为mNGS技术流程的质量控制的重要节点。且相比有菌部位的标本，无菌部位的标本（静脉血、脑脊液、胸腔积液、组织）具有更高的临床价值，应尽量送检无菌部位的标本。因此，本文通过对mNGS无菌部位临床样本处理方法进行综述，着重阐述无菌部位样本的特性及处理方法，以期为医疗工作者及科研人员提供详实的参考信息。更多还原

【Abstract】 Pathogen metagenomic high-throughput sequencing(mNGS)does not rely on traditional microbial cμLture,and directly performs high-throughput sequencing of nucleic acids in clinical samples,which has the advantages of unbiased,wide coverage,and rapidity. The detection of more pathogenic microorganisms(including viruses,bacteria,fungi,and parasites)in clinical samples has played a key role in the field of severe infections,especially those caused by difficμLt and rare diseases. However,infectious clinical samples are diverse,and there is no standard specification for sample pretreatment and nucleic acid extraction methods. The consensus of various experts also pointed out that sample pre-processing and nucleic acid extraction are important nodes in the quality control of the mNGS technical process. And compared with the specimens from the bacterial site,the specimens from the sterile site(venous blood,cerebrospinal fluid,pleural effusion,tissue)have higher clinical value and shoμLd be submitted for examination as far as possible. Therefore,this article summarizes the clinical sample processing methods of m NGS sterile parts,focusing on the characteristics and processing methods of the sterile parts samples,in order to provide detailed reference information for medical workers and scientific researchers.更多还原