【摘要】 目的：建立HPLC一测多评法同时测定胃疡灵颗粒中肉桂酸、桂皮醛、毛蕊异黄酮葡萄糖苷、芒柄花苷、芒柄花素、6-姜辣素、8-姜酚和10-姜酚的含量。方法：采用HPLC法，以Agilent SB-C₁₈柱（250 mm×4.6 mm, 5μm）为色谱柱，流动相A为乙腈-甲醇（9∶1）,流动相B为0.1%磷酸溶液，梯度洗脱（0～13 min, 28%A;13～24 min, 28%→35%A;24～39 min, 35%→56%A;39～53 min, 56%→85%A;53～60 min, 85%→28%A）,流速为1.1 ml/min,检测波长为280 nm,柱温为25℃。以毛蕊异黄酮葡萄糖苷为内参物，建立其他7个成分的相对校正因子，计算各成分含量。结果：肉桂酸、桂皮醛、毛蕊异黄酮葡萄糖苷、芒柄花苷、芒柄花素、6-姜辣素、8-姜酚和10-姜酚分别在0.59～11.80、7.47～149.40、2.18～43.60、1.91～38.20、4.49～89.80、3.87～77.40、0.94～18.80、1.46～29.20μg/ml（r≥0.999 1）范围内线性关系良好；平均加样回收率分别为（96.89±1.04）%、（100.05±0.64）%、（99.52±1.18）%、（98.51±1.30）%、（99.38±0.77）%、（98.29±0.88）%、（97.72±1.26）%和（97.93±1.01）%（n=9）;肉桂酸、桂皮醛、芒柄花苷、芒柄花素、6-姜辣素、8-姜酚和10-姜酚的相对校正因子分别为0.826 3、0.849 0、0.972 0、1.200 5、1.276 6、0.720 2和0.874 9,一测多评法计算值和外标法实测值无明显差异。结论：本研究建立的HPLC一测多评法操作便捷、结果准确，可用于胃疡灵颗粒的质量控制。更多还原

【Abstract】 Objective:To establish a high performance liquid chromatography quantitative analysis of multi-components by single marker（HPLC-QAMS） for simultaneous determination of cinnamic acid, cinnamaldehyde, calycosin-7-O-β-D-glucopyranoside, ononin, formononetin, 6-gingerol, 8-gingerol and 10-gingerol in Weiyangling granules.Methods:An HPLC method was established with Agilent SB-C₁₈（250 mm×4.6 mm, 5 μm） as a separate column.Acetonitrile-methanol（9∶1,phase A） and 0.1% phosphoric acid（phase B） were used as a mobile phase, and the flow rate was 1.1 ml/min with a gradient elution（0-13 min, 28%A; 13-24 min, 28%→35%A;24-39 min, 35%→56%A; 39-53 min, 56%→85%A;53-60 min, 85%→28%A）.The detection wavelength was set at 280 nm and the column temperature was 25 ℃.With calycosin-7-O-β-D-glucopyranoside as an internal standard, the relative correction factors of the other seven components were established and the contents of various components were calculated.Results:Cinnamic acid, cinnamaldehyde, calycosi-7-O-β-D-glucopyranoside, ononin, formononetin, 6-gingerol, 8-gingerol and 10-gingerol showed good linear relationships within the ranges of 0.59-11.80,7.47-149.40,2.18-43.60,1.91-38.20,4.49-89.80,3.87-77.40,0.94-18.80 and 1.46-29.20 μg/ml（r≥0.999 1）,with average recovery rates of（96.89±1.04）%,（100.05±0.64）%,（99.52±1.18）%,（98.51±1.30）%,（99.38±0.77）%,（98.29±0.88）%,（97.72±1.26）% and（97.93±1.01）%（n=9） respectively.The relative correction factors of cinnamic acid, cinnamaldehyde, ononin, formononetin, 6-gingerol, 8-gingerol and 10-gingerol were respectively 0.826 3,0.849 0,0.972 0,1.200 5,1.276 6,0.720 2 and 0.874 9.There were no significant differences between the calculated values by HPLC-QAMS method and the measured values by external standard method.Conclusion:The HPLC-QAMS method established by our study is simple and accurate, which could be used for the quality control of Weiyangling granules.更多还原