【摘要】 金龟子绿僵菌(Metarhiziumanisopliae)是一种广谱的昆虫病原菌，可作为真菌杀虫剂防治多种害虫。绿僵菌与昆虫的相互作用十分复杂，诸多因素均可影响其侵染效果。本研究筛选出一株对斜纹夜蛾(Spodoptera litoptera)有较好杀虫效果的绿僵菌菌株，随后分析紫外线-B(UV-B)胁迫处理的斜纹夜蛾被真菌感染后的死亡率、血细胞数量、体内吞噬作用、血浆抗真菌活性等。结果表明，UV-B前期照射能显著降低斜纹夜蛾幼虫被绿僵菌侵染的死亡率。UV-B照射斜纹夜蛾幼虫0.5 h后用绿僵菌侵染处理4～8 h，免疫反应相关的指标明显增加。说明昆虫对昆虫病原真菌的敏感性受紫外线胁迫的影响，紫外线能改变昆虫体内的免疫反应，促使昆虫抵御病原真菌的侵染。更多还原

【Abstract】 Metarhizium anisopliae is a broad spectrum insect pathogen, which can be used as a fungal insecticide to control various insect pests. The interaction between M. anisopliae and insects is very complex, and various factors may affect its infection effect. In this study, a strain of M. anisopliae with good insecticidal effect was obtained. The mortality, haemocyte number in haemolymph, phagocytosis and anti-fungal activity in plasma were analyzed in fungal infected Spodoptera litoptera after UV-B stress treatment. The results showed that within 4 to 8 h after treatment(UV-B stress for 0.5 h followed by infection with M. anisopliae), there was a significant increase in indicators related to the immune response. These results indicated that the sensitivity of insects to entomopathogenic fungi was affected by UV-B stress, and UV-B could change the immune response of insects,making them resistant to pathogenic fungi.更多还原